Rabbit MAGI-2 Polyclonal Antibody | anti-MAGI-2 antibody

MAGI-2 (E255) Polyclonal Antibody

Cat. Num. AAA3003009

• Gene Names: MAGI2; AIP1; AIP-1; ARIP1; SSCAM; MAGI-2; NPHS15; ACVRIP1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MAGI-2; Polyclonal Antibody; MAGI-2 (E255) Polyclonal Antibody; Membrane-associated guanylate kinase; WW and PDZ domain-containing protein 2; Atrophin-1-interacting protein 1; AIP-1; Atrophin-1-interacting protein A; Membrane-associated guanylate kinase inverted 2; MAGI2; ACVRINP1; AIP1; KIAA0705; anti-MAGI-2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MAGI-2 (E255) polyclonal antibody detects endogenous levels of MAGI-2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1455

• Applicable Applications for anti-MAGI-2 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 224-276 of Human MAGI-2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Related Product Information for anti-MAGI-2 antibody

The closely related MAGUK proteins, MAGI-1, MAGI-2 and MAGI-3 (membrane associated guanylate kinase inverted-1 and 2), likewise contain the GuK domain and five PDZ domains; however, the SH3 domain is replaced with a WW domain. The transcripts of MAGI-1 are alternatively spliced to produce three distinct proteins having unique carboxy-terminals. Two variants, MAGI-1a and MAGI-1b, are associated with the membrane and cytosolic fractions and are primarily expressed in the brain. The third isoform, MAGI-1c, encodes for a nuclear localization signal that localizes MAGI-1c to the nucleus, and it is primarily expressed in the liver and kidney. MAGI-2 and MAGI-3 are localized to the plasma membrane,

NCBI and Uniprot Product Information

• NCBI GI #: 27436957
• NCBI GeneID: 9863
• NCBI Accession #: NP_036433.2
• NCBI GenBank Nucleotide #: NP_036433.2
• UniProt Accession #: Q86UL8
• Molecular Weight: ~ 159kDa
• NCBI Official Full Name: membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: membrane associated guanylate kinase, WW and PDZ domain containing 2
• NCBI Official Symbol: MAGI2
• NCBI Official Synonym Symbols: AIP1; AIP-1; ARIP1; SSCAM; MAGI-2; NPHS15; ACVRIP1
• NCBI Protein Information: membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
• UniProt Protein Name: Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
• UniProt Gene Name: MAGI2
• UniProt Synonym Gene Names: ACVRINP1; AIP1; KIAA0705; AIP-1; MAGI-2
• UniProt Entry Name: MAGI2_HUMAN

NCBI Description

The protein encoded by this gene interacts with atrophin-1. Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. This encoded protein is characterized by two WW domains, a guanylate kinase-like domain, and multiple PDZ domains. It has structural similarity to the membrane-associated guanylate kinase homologue (MAGUK) family. [provided by RefSeq, Jul 2008]

Uniprot Description

AIP1: a scaffold molecule at synaptic junctions that apparently couples neurotransmitter receptors and cell adhesion proteins. Contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. Contains two WW domains, a guanylate kinase-like domain, and multiple PDZ domains. It has structural similarity to the membrane-associated guanylate kinase homologue (MAGUK) family. This protein is brain specific, where it appears to be widely expressed in both neurons and glia. Enhances the ability of PTEN to suppress AKT1 activation. Two splice-variant isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: protein complex; tight junction; perinuclear region of cytoplasm; cytoplasm; postsynaptic density; dendrite; late endosome; plasma membrane; synapse; nucleus

Molecular Function: signal transducer activity; protein binding; receptor signaling complex scaffold activity; beta-1 adrenergic receptor binding; SMAD binding; phosphatase binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; negative regulation of activin receptor signaling pathway; negative regulation of cell proliferation; negative regulation of protein kinase B signaling cascade; protein heterooligomerization; positive regulation of receptor internalization; negative regulation of cell migration; receptor clustering; cytoplasmic transport