Rabbit anti-Human MAFK Polyclonal Antibody | anti-MAFK antibody

MAFK, CT (v-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog K (Avian), MAFK Protein, FLJ32205, MGC71717, NFE2U, P18) (PE)

Cat. Num. AAA6486175

• Gene Names: SEPTIN9; MSF; MSF1; NAPB; SEPT9; SINT1; PNUTL4; SeptD1; AF17q25
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MAFK; Polyclonal Antibody; CT (v-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog K (Avian); MAFK Protein; FLJ32205; MGC71717; NFE2U; P18) (PE); anti-MAFK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAFK.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 568

• Applicable Applications for anti-MAFK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MAFK (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(The anti-SEPT9 Pab is used in Western blot to detect SEPT9 in Jurkat cell lysate.)

Immunohistochemistry (IHC)

(SEPT9 Antibody (A555) immunohistochemistry analysis in formalin fixed and paraffin embedded kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEPT9 Antibody (A555) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SEPT9 Antibody (A555) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-MAFK antibody

Antibodies; Transcription Factors

References

1. Proc. Natl. Acad. Sci. USA 96:6428-6433(1999). 2. Cancer Res. 60: 4729-4734, 2000. 3. Oncogene 20: 5930-5939, 2001. 4. Kristi L, et al. Cancer Res., Dec 2009; 69: 9301-9305. (E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 116256489
• NCBI GeneID: 10801
• NCBI Accession #: NP_006631.2
• NCBI GenBank Nucleotide #: NP_006631.2
• UniProt Accession #: Q9UHD8
• Molecular Weight: 63.633
• NCBI Official Full Name: septin-9 isoform c
• NCBI Official Synonym Full Names: septin 9
• NCBI Official Symbol: SEPTIN9
• NCBI Official Synonym Symbols: MSF; MSF1; NAPB; SEPT9; SINT1; PNUTL4; SeptD1; AF17q25
• NCBI Protein Information: septin-9
• UniProt Protein Name: Septin-9
• Protein Family: Transcription factor
• UniProt Gene Name: SEPT9
• UniProt Synonym Gene Names: KIAA0991; MSF; MLL septin-like fusion protein; Ov/Br septin
• UniProt Entry Name: SEPT9_HUMAN

NCBI Description

This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2009]

Uniprot Description

Filament-forming cytoskeletal GTPase (). May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.