Rabbit anti-Human MAFB Polyclonal Antibody | anti-MAFB antibody

MAFB, CT (MAFB, KRML, Transcription factor MafB, V-maf musculoaponeurotic fibrosarcoma oncogene homolog B) (Biotin)

Cat. Num. AAA6317991

• Gene Names: MAFB; KRML; MCTO
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MAFB; Polyclonal Antibody; CT (MAFB; KRML; Transcription factor MafB; V-maf musculoaponeurotic fibrosarcoma oncogene homolog B) (Biotin); V-maf musculoaponeurotic fibrosarcoma oncogene homolog B; anti-MAFB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAFB.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MAFB antibody: Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 266-292 in the C-terminal region of human MAFB, UniProt Accession #Q9Y5Q3. Species sequence homology: monkey, mouse, rat, xenopus
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane) usingMBS6004031. This demonstrates that MBS6004031 detected the MAFB protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Jurkat cells using MBS6004031 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MAFB antibody

The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns. [provided by RefSeq].

Product Categories/Family for anti-MAFB antibody

Antibodies; Transcription Factors

References

1. Kim, H., et al. Nat. Immunol. 11(8):743-750(2010). 2. Beaty, T.H., et al. Nat. Genet. 42(6):525-529(2010). 3. Vatsveen, T.K., et al. Leuk. Res. 33(12):1670-1677(2009). 4. Zanocco-Marani, T., et al. Exp. Cell Res. 315(11):1798-1808(2009). 5. Kathiresan, S., et al. Nat. Genet. 41(1):56-65(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 23308699
• NCBI GeneID: 9935
• NCBI Accession #: NP_005452.2
• NCBI GenBank Nucleotide #: NM_005461.4
• UniProt Accession #: Q9Y5Q3; Q9H1F1; B3KNE1
• Molecular Weight: 35,792 Da
• NCBI Official Full Name: transcription factor MafB
• NCBI Official Synonym Full Names: v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B
• NCBI Official Symbol: MAFB
• NCBI Official Synonym Symbols: KRML; MCTO
• NCBI Protein Information: transcription factor MafB; Kreisler maf-related leucine zipper homolog; MAFB/Kreisler basic region/leucine zipper transcription factor
• UniProt Protein Name: Transcription factor MafB
• Protein Family: Transcription factor
• UniProt Gene Name: MAFB
• UniProt Synonym Gene Names: KRML; Maf-B
• UniProt Entry Name: MAFB_HUMAN