Rabbit MAD2L1 Polyclonal Antibody | anti-MAD2L1 antibody

MAD2L1 (Mitotic Spindle Assembly Checkpoint Protein MAD2A, MAD2-like Protein 1, MAD2, HSMAD2) (FITC)

Cat. Num. AAA6425168

• Gene Names: MAD2L1; MAD2; HSMAD2
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: MAD2L1; Polyclonal Antibody; MAD2L1 (Mitotic Spindle Assembly Checkpoint Protein MAD2A; MAD2-like Protein 1; MAD2; HSMAD2) (FITC); anti-MAD2L1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes MAD2L1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-MAD2L1 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human MAD2L1.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MAD2L1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human rectal cancer using MAD2L1 antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat testis using MAD2L1 antibody at dilution of 1:100 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using MAD2L1 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-MAD2L1 antibody

Antibodies; Cell Division Cycle (CDC)

NCBI and Uniprot Product Information

• NCBI GI #: 4505067
• NCBI GeneID: 4085
• NCBI Accession #: NP_002349.1
• NCBI GenBank Nucleotide #: NM_002358.3
• UniProt Accession #: Q13257; Q53F56; Q548X9; Q6IRW7
• Molecular Weight: 23,510 Da
• NCBI Official Full Name: mitotic spindle assembly checkpoint protein MAD2A
• NCBI Official Synonym Full Names: MAD2 mitotic arrest deficient-like 1 (yeast)
• NCBI Official Symbol: MAD2L1
• NCBI Official Synonym Symbols: MAD2; HSMAD2
• NCBI Protein Information: mitotic spindle assembly checkpoint protein MAD2A; MAD2-like protein 1; mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
• UniProt Protein Name: Mitotic spindle assembly checkpoint protein MAD2A
• Protein Family: MAD2L1-binding protein
• UniProt Gene Name: MAD2L1
• UniProt Synonym Gene Names: MAD2; HsMAD2
• UniProt Entry Name: MD2L1_HUMAN

NCBI Description

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]

Uniprot Description

MAD2L1: required for the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. Inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Interacts with Cdc20, MAD2L1BP and with ADAM17/TACE

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: kinetochore; spindle pole; perinuclear region of cytoplasm; nuclear pore; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity

Biological Process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; negative regulation of mitotic cell cycle; mitotic cell cycle; negative regulation of protein catabolic process; negative regulation of apoptosis