Rabbit anti-Human Macrophage Inflammatory Protein 3 Polyclonal Antibody | anti-CCL19 antibody
Macrophage Inflammatory Protein 3, Human (MIP-3)
Purified by antigen affinity chromatography
Purified by antigen affinity chromatography
ELISA: To detect hMIP-3 by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant hMIP-3.
Western Blot: To detect hMIP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Reconstitute in 100ul of water for each 100ug of antibody to obtain a solution of 1mg/ml antibody in PBS. This solution can be diluted into other buffered solutions or stored at -20 degree C for future use. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 degree C. When reconstituted in sterile water to a concentration of ~0.2mg/ml the antibody is stable for at least six weeks at 2-4 degree C.
NCBI and Uniprot Product Information
NCBI Description
This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Jul 2008]
Uniprot Description
CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 9p13
Cellular Component: extracellular space; extracellular region
Molecular Function: CCR chemokine receptor binding; chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; CCR7 chemokine receptor binding
Biological Process: formation of immunological synapse; positive regulation of interleukin-12 production; positive regulation of dendritic cell antigen processing and presentation; cell maturation; positive regulation of JNK cascade; positive regulation of endocytosis; positive regulation of NF-kappaB import into nucleus; positive regulation of interleukin-1 beta secretion; positive regulation of receptor-mediated endocytosis; activation of JNK activity; dendritic cell chemotaxis; positive regulation of T cell proliferation; cell communication; establishment of T cell polarity; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of chemotaxis; response to virus; positive regulation of phosphoinositide 3-kinase activity; positive regulation of tumor necrosis factor production; cellular calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; myeloid dendritic cell chemotaxis; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; T cell costimulation; immune response; positive regulation of T-helper 1 cell differentiation
Research Articles on CCL19
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Product Notes
The CCL19 ccl19 (Catalog #AAA626987) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Macrophage Inflammatory Protein 3, Human (MIP-3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Macrophage Inflammatory Protein 3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Neutralization. Suitable for use in Neutralization, ELISA, Western Blotting. ELISA: To detect hMIP-3 by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant hMIP-3. Western Blot: To detect hMIP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Reconstitute in 100ul of water for each 100ug of antibody to obtain a solution of 1mg/ml antibody in PBS. This solution can be diluted into other buffered solutions or stored at -20 degree C for future use. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 degree C. When reconstituted in sterile water to a concentration of ~0.2mg/ml the antibody is stable for at least six weeks at 2-4 degree C. Researchers should empirically determine the suitability of the CCL19 ccl19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Macrophage Inflammatory Protein 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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