Rabbit anti-Rat Macrophage Inflammatory Protein 1 alpha Polyclonal Antibody | anti-CCL3 antibody

Macrophage Inflammatory Protein 1 alpha (MIP-1 alpha, CCL3, LD78 alpha) (Biotin)

Cat. Num. AAA615278

• Gene Names: CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Macrophage Inflammatory Protein 1 alpha; Polyclonal Antibody; Macrophage Inflammatory Protein 1 alpha (MIP-1 alpha; CCL3; LD78 alpha) (Biotin); Anti -Macrophage Inflammatory Protein 1 alpha (MIP-1 alpha; anti-CCL3 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Macrophage Inflammatory Protein 1 alpha.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS. Labeled with Biotin.

• Applicable Applications for anti-CCL3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Direct ELISA: 0.15-0.3ug/ml using 100ul/well antibody solution. Allows the detection of at least 0.2ng/well of recombinant rat MIP-1a.; Western Blot: 0.1-0.2ug/ml. The dectection limit is 1.5-3ng/lane, under reducing or non-reducing conditions.
• Immunogen: Highly purified (>98%) recombinant rat Macrophage Inflammatory Protein 1 alpha (MIP-1 alpha).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CCL3 antibody

Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1 alpha and MIP-1 beta are active on human and murine hematopoietic cells.

Product Categories/Family for anti-CCL3 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 2905628
• NCBI GeneID: 6348
• UniProt Accession #: P10147; Q14745
• Molecular Weight: 10,085 Da
• NCBI Official Full Name: macrophage inflammatory protein 1 alpha
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 3
• NCBI Official Symbol: CCL3
• NCBI Official Synonym Symbols: MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
• NCBI Protein Information: C-C motif chemokine 3; SIS-beta; PAT 464.1; OTTHUMP00000163953; G0/G1 switch regulatory protein 19-1; macrophage inflammatory protein 1-alpha; tonsillar lymphocyte LD78 alpha protein; small inducible cytokine A3 (homologous to mouse Mip-1a)
• UniProt Protein Name: C-C motif chemokine 3
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL3
• UniProt Synonym Gene Names: G0S19-1; MIP1A; SCYA3
• UniProt Entry Name: CCL3_HUMAN

NCBI Description

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.

Uniprot Description

CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular; cytosol

Molecular Function: identical protein binding; CCR1 chemokine receptor binding; protein binding; chemokine activity; calcium-dependent protein kinase C activity; kinase activity; CCR5 chemokine receptor binding; protein kinase activity; chemoattractant activity; phospholipase activator activity

Biological Process: positive regulation of catalytic activity; negative regulation of osteoclast differentiation; exocytosis; response to toxin; behavior; positive regulation of calcium-mediated signaling; positive regulation of interleukin-1 beta secretion; chemotaxis; protein amino acid phosphorylation; negative regulation of bone mineralization; regulation of cell shape; monocyte chemotaxis; positive chemotaxis; cell-cell signaling; positive regulation of neuron apoptosis; calcium ion transport; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; calcium-mediated signaling; MAPKKK cascade; cytoskeleton organization and biogenesis; positive regulation of tumor necrosis factor production; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; macrophage chemotaxis; cellular calcium ion homeostasis; osteoblast differentiation; cell activation; positive regulation of protein kinase B signaling cascade; eosinophil chemotaxis; eosinophil degranulation; regulation of sensory perception of pain; positive regulation of calcium ion transport; astrocyte cell migration; positive regulation of cell migration; positive regulation of inflammatory response

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To