Rabbit anti-Mouse Macrophage Inflammatory Protein 1 gamma Polyclonal Antibody | anti-MIP-1g antibody
Macrophage Inflammatory Protein 1 gamma, Murine (MIP-1g) (APC)
Reactivity
Mouse
Applications
ELISA, Western Blot, Neutralization Assay
Purity
Purified by immobilized antigen affinity chromatography.
Synonyms
Macrophage Inflammatory Protein 1 gamma; Polyclonal Antibody; Murine (MIP-1g) (APC); anti-MIP-1g antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Macrophage Inflammatory Protein-1 gamma, mouse (MIP-1 gamma)
Purity/Purification
Purified by immobilized antigen affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-MIP-1g antibody
ELISA (EIA), Western Blot (WB), Neutralization
Application Notes
ELISA: Detection by direct ELISA (using 100ul/well antibody solution), requires a concentration of at least 0.5ug/ml of antibody. This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well.
WB: Use at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity (50ng/ml), a concentration of 1.75-3.0ug/ml of this antibody is required.
Applications are based on unconjugated antibody.
WB: Use at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity (50ng/ml), a concentration of 1.75-3.0ug/ml of this antibody is required.
Applications are based on unconjugated antibody.
Immunogen
Highly purified (>98%) recombinant antigen.
Conjugate
APC
Reconstitution
Lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 degree C. When reconstituted in sterile water to a concentration of >=0.2mg/ml the antibody is stable for at least six weeks at 2-4 degree C.
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Allophycocyanin (APC) is light sensitive.
Product Categories/Family for anti-MIP-1g antibody
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Product Notes
The MIP-1g (Catalog #AAA6485924) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Macrophage Inflammatory Protein 1 gamma, Murine (MIP-1g) (APC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Macrophage Inflammatory Protein 1 gamma can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Neutralization. ELISA: Detection by direct ELISA (using 100ul/well antibody solution), requires a concentration of at least 0.5ug/ml of antibody. This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well. WB: Use at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity (50ng/ml), a concentration of 1.75-3.0ug/ml of this antibody is required. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MIP-1g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Macrophage Inflammatory Protein 1 gamma, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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