Goat anti-Human, Mouse MAC-2 / Galectin 3 Polyclonal Antibody | anti-LGALS3 antibody

Goat Polyclonal to Mouse MAC-2 / Galectin 3

Cat. Num. AAA248591

• Gene Names: LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MAC-2 / Galectin 3; Polyclonal Antibody; Goat Polyclonal to Mouse MAC-2 / Galectin 3; Anti-MAC-2 / Galectin 3 Antibody (Internal) IHC-plus; 35 kd lectin; 35 kDa lectin; CBP35; GAL3; Galactoside-binding protein; GALIG; IgE-binding protein; Laminin-binding protein; Gal-3; GALBP; Mac-2 antigen; L31; MAC2; CBP 35; Galactose-specific lectin 3; Galectin-3; L-31; Lectin L-29; Galectin 3; Mouse MAC-2; anti-LGALS3 antibody

• Host: Goat
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Mouse LGALS3 / Galectin-3. Reported variants represent identical protein: NP_001139425.1, NP_034835.1
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 250

• Applicable Applications for anti-LGALS3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:4000), IHC-P (10 ug/ml), WB (0.5 - 2 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 26kD band observed in Mouse Heart lysates (calculated MW of 27.4kD according to NP_034835.1). Recommended concentration: 0.5-2 ug/ml.
• Target Species: Mouse
• Immunogen Description: Synthetic peptide C-NANRIVLDFRRGND from an internal region of mouse LGALS3 / Galectin-3 (NP_034835.1). Percent identity with other species by BLAST analysis: Mouse (100%), Human (86%), Gorilla (86%), Gibbon (86%), Elephant (86%).
• Immunogen Type: Synthetic peptide
• Immunogen: MAC-2 / Galectin 3 antibody was raised against synthetic peptide C-NANRIVLDFRRGND from an internal region of mouse LGALS3 / Galectin-3 (NP_034835.1). Percent identity with other species by BLAST analysis: Mouse (100%), Human (86%), Gorilla (86%), Gibbon (86%), Elephant (86%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-LGALS3 / Galectin 3 antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(LGALS3 antibody (0.5 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 115430223
• NCBI GeneID: 3958
• NCBI Accession #: NP_002297.2
• NCBI GenBank Nucleotide #: NM_002306.3
• UniProt Accession #: P17931; Q16005; Q6IBA7; Q96J47; B2RC38
• Molecular Weight: 26,152 Da
• NCBI Official Full Name: galectin-3 isoform 1
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 3
• NCBI Official Symbol: LGALS3
• NCBI Official Synonym Symbols: L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
• NCBI Protein Information: galectin-3; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35
• UniProt Protein Name: Galectin-3
• UniProt Gene Name: LGALS3
• UniProt Synonym Gene Names: MAC2; Gal-3; CBP 35; GALBP
• UniProt Entry Name: LEG3_HUMAN

NCBI Description

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Uniprot Description

Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.

Protein type: Motility/polarity/chemotaxis; Cell surface; Extracellular matrix

Chromosomal Location of Human Ortholog: 14q22.3

Cellular Component: extracellular matrix; spliceosome; proteinaceous extracellular matrix; extracellular space; membrane; mitochondrial inner membrane; cytoplasm; plasma membrane; immunological synapse; nucleus; external side of plasma membrane

Molecular Function: protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; macrophage chemotaxis; monocyte chemotaxis; positive chemotaxis; epithelial cell differentiation; eosinophil chemotaxis; innate immune response; mRNA processing; skeletal development