Rabbit M-cadherin Polyclonal Antibody | anti-A108 antibody

M-cadherin (A108) Polyclonal Antibody

Cat. Num. AAA3002312

• Gene Names: CDH15; CDH3; CDHM; MCAD; MRD3; CDH14
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: M-cadherin; Polyclonal Antibody; M-cadherin (A108) Polyclonal Antibody; Cadherin-15; Muscle cadherin; Cadherin-14; CDH15; CDH14; CDH3; anti-A108 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: M-cadherin (A108) polyclonal antibody detects endogenous levels of M-cadherin protein..
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 814

• Applicable Applications for anti-A108 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 80-130 of Human M-cadherin.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of M-cadherin (A108) Antibody at 1:500 dilutionLane1:MG63 whole cell lysate (30ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:The Brain tissue lysate of Mouse (40ug) Lane4:The Brain tissue lysate of Rat (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of M-cadherin (A108) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-A108 antibody

Cadherins are a multigene family of Ca++-dependent cell adhesion molecules. They are transmembrane glycoproteins consisting of an extracellular domain, which mediates Ca++-dependent intercellular adhesion by homophilic interactions, a transmembrane region and a cytoplasmic domain. The extracellular domain is divided into a series of subdomains designated EC1-EC5. Homolgies between different members of the cadherin family are most prominent in the cytoplasmic domain and in EC1 and EC2 and much less so in EC5 of the extracellular domain and in the transmembrane region. The binding properties and specificities of the adhesive function are located in the N-terminal part of the molecules. Four members of the cadherin family have been identified and molecularly cloned from mammalian cells. These include the neuronal (N), epithelial (E), placental (P) and muscle (M) cadherins. M-cadherin is not found in fibroblasts but is expressed at low level in myoblasts and is upregulated following induction of myotube formation, suggesting a specific function in skeletal muscle cell differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 4826669
• NCBI GeneID: 1013
• NCBI Accession #: NP_004924.1
• NCBI GenBank Nucleotide #: NP_004924.1
• UniProt Accession #: P55291
• Molecular Weight: ~ 91kDa
• NCBI Official Full Name: cadherin-15 preproprotein
• NCBI Official Synonym Full Names: cadherin 15
• NCBI Official Symbol: CDH15
• NCBI Official Synonym Symbols: CDH3; CDHM; MCAD; MRD3; CDH14
• NCBI Protein Information: cadherin-15
• UniProt Protein Name: Cadherin-15
• UniProt Gene Name: CDH15
• UniProt Synonym Gene Names: CDH14; CDH3; M-cadherin
• UniProt Entry Name: CAD15_HUMAN

NCBI Description

This gene is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. The protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH15: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation. A chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24). Defects in CDH15 are the cause of mental retardation autosomal dominant type 3 (MRD3). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: integral to membrane; plasma membrane; caveola; neuromuscular junction

Molecular Function: calcium ion binding

Biological Process: intercellular junction assembly and maintenance; muscle cell differentiation; positive regulation of muscle cell differentiation; homophilic cell adhesion; cell adhesion

Disease: Mental Retardation, Autosomal Dominant 3