Rabbit M-4 Muscarinic receptor IgG fraction Polyclonal Antibody
M-4 Muscarinic receptor IgG fraction
Applications
Immunoblot, Western Blot, Western Blot
Synonyms
M-4 Muscarinic receptor IgG fraction; Polyclonal Antibody; Muscarinic M 4 Receptor; M 4 Receptor (469-478); IgG Fraction Rabbit Anti-Muscarinic M4 Receptor Serum; anti-M-4 Muscarinic receptor IgG fraction antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Polypeptide ::: % Cross Reactivity
M4 Muscarinic Receptor (469-478) ::: 100
M4 Muscarinic Receptor ::: 70
M4 Muscarinic Receptor (469-478) ::: 100
M4 Muscarinic Receptor ::: 70
Form/Format
Antiserum Specificity
Applicable Applications for anti-M-4 Muscarinic receptor IgG fraction antibody
Immunocytochemical Staining, Western Immunoblot, Western Blot
Application Notes
Immunocytochemistry: The purified IgG has been found to stain PLP fixed cultured cells known to express the muscarinic M4 receptor at a concentration of 50 ug/ml.
Western Blot: Western immunoblots using whole rat brain homogenate and cultured cells known to express the M4 have been successful at a concentration of 80 ug/ml.
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the IgG fraction after diluting to at least 80 ug/ml (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours. Wash x 3 with TBS/Tween-20.
4. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
5. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Western Blot: Western immunoblots using whole rat brain homogenate and cultured cells known to express the M4 have been successful at a concentration of 80 ug/ml.
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the IgG fraction after diluting to at least 80 ug/ml (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours. Wash x 3 with TBS/Tween-20.
4. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
5. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Related Product Information for anti-M-4 Muscarinic receptor IgG fraction antibody
This IgG fraction was prepared by precipitation. dialysis and chromatography from an antiserum that was raised in a rabbit which was immunized with a peptide analogue of the carboxyl terminal of the M4 receptor covalently attached onto a carrier protein. The antiserum has been shown to be specific for the COOH terminal of the M4 receptor and has been characterized by western blotting. ELISA. and cell staining techniques. This IgG fraction is suitable for immunocytochemical and western immunoblotting detection of the receptor. Dilute the lyophilized antibody with 0.1 ml of 10 mg/ml BSA in PBS for the equivalent concentration in whole antiserum. or with additional buffer for more dilute antibody. This vial contains 3.0 mg of purified IgG.
Product Categories/Family for anti-M-4 Muscarinic receptor IgG fraction antibody
Similar Products
Product Notes
The M-4 Muscarinic receptor IgG fraction (Catalog #AAA352160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's M-4 Muscarinic receptor IgG fraction can be used in a range of immunoassay formats including, but not limited to, Immunocytochemical Staining, Western Immunoblot, Western Blot. Immunocytochemistry: The purified IgG has been found to stain PLP fixed cultured cells known to express the muscarinic M4 receptor at a concentration of 50 ug/ml. Western Blot: Western immunoblots using whole rat brain homogenate and cultured cells known to express the M4 have been successful at a concentration of 80 ug/ml. Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer. 2. Wash x 2 with TBS/Tween-20. 3. Apply the IgG fraction after diluting to at least 80 ug/ml (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours. Wash x 3 with TBS/Tween-20. 4. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques. 5. Wash x 4 for 5 minute/wash with TBS/Tween-20. 7. Develop color using the enhanced DAB reaction. Researchers should empirically determine the suitability of the M-4 Muscarinic receptor IgG fraction for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "M-4 Muscarinic receptor IgG fraction, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
