Rabbit anti-Human, Mouse LYVE1 Polyclonal Antibody | anti-LYVE1 antibody

LYVE1, NT (Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1, Cell Surface Retention Sequence-binding Protein 1, CRSBP-1, Hyaluronic Acid Receptor, Extracellular Link Domain-containing Protein 1, LYVE-1, CRSBP1, HAR, XLKD1, UNQ230/PRO263) (AP)

Cat. Num. AAA6485704

• Gene Names: LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: LYVE1; Polyclonal Antibody; NT (Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1; Cell Surface Retention Sequence-binding Protein 1; CRSBP-1; Hyaluronic Acid Receptor; Extracellular Link Domain-containing Protein 1; LYVE-1; CRSBP1; HAR; XLKD1; UNQ230/PRO263) (AP); anti-LYVE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human XLKD1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-LYVE1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human XLKD1 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-XLKD1 N-term Pab is used in Western blot to detect XLKD1 in mouse liver tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with theMBS623417, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS623417 for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(XLKD1 Antibody (N-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the XLKD1 antibody detected the XLKD1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of XLKD1 (arrow) using rabbit polyclonal XLKD1 Antibody (C61). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XLKD1 gene.)

Product Categories/Family for anti-LYVE1 antibody

Antibodies; Receptors

References

1. Jackson, D.G., Trends Cardiovasc. Med. 13(1):1-7 (2003). 2. Cursiefen, C., et al., Invest. Ophthalmol. Vis. Sci. 43(7):2127-2135 (2002). 3. Cunnick, G.H., et al., Biochem. Biophys. Res. Commun. 288(4):1043-1046 (2001). 4. Mouta Carreira, C., et al., Cancer Res. 61(22):8079-8084 (2001). 5. Banerji, S., et al., J. Cell Biol. 144(4):789-801 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 40549451
• NCBI GeneID: 10894
• NCBI Accession #: NP_006682
• NCBI GenBank Nucleotide #: NM_006691.3
• UniProt Accession #: Q9Y5Y7; Q8TC18; Q9UNF4
• Molecular Weight: 35,213 Da
• NCBI Official Full Name: lymphatic vessel endothelial hyaluronic acid receptor 1
• NCBI Official Synonym Full Names: lymphatic vessel endothelial hyaluronan receptor 1
• NCBI Official Symbol: LYVE1
• NCBI Official Synonym Symbols: HAR; XLKD1; LYVE-1; CRSBP-1
• NCBI Protein Information: lymphatic vessel endothelial hyaluronic acid receptor 1
• UniProt Protein Name: Lymphatic vessel endothelial hyaluronic acid receptor 1
• Protein Family: Lymphatic vessel endothelial hyaluronic acid receptor
• UniProt Gene Name: LYVE1
• UniProt Synonym Gene Names: CRSBP1; HAR; XLKD1; LYVE-1; CRSBP-1
• UniProt Entry Name: LYVE1_HUMAN

NCBI Description

This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

XLKD1: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; hyaluronic acid binding; receptor activity

Biological Process: anatomical structure morphogenesis; glycosaminoglycan metabolic process; transport; cell-matrix adhesion; carbohydrate metabolic process; response to wounding; pathogenesis; cell motility; signal transduction; hyaluronan metabolic process; hyaluronan catabolic process