Western Blot (WB)
(Western Blot of human cerebella supernatant (40ug) using MBS611438.)
Rabbit Lysophospholipase D Polyclonal Antibody
Lysophospholipase D (LysoPLD)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Lysophospholipase D; Polyclonal Antibody; Lysophospholipase D (LysoPLD); Anti -Lysophospholipase D (LysoPLD); anti-Lysophospholipase D antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat LysoPLD. Species Crossreactivity: human and mouse.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from TBS, pH 7.4. Reconstitute with 500ul sterile ddH2O.
Applicable Applications for anti-Lysophospholipase D antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot and Immunofluorescence.
Dilution: Western Blot: 5ug/ml
Immunofluorescence (IF-IC): 2ug/ml
Dilution: Western Blot: 5ug/ml
Immunofluorescence (IF-IC): 2ug/ml
Immunogen
Synthetic peptide corresponding to aa573-588, KNKLEELNKRLHTKGS from rat Lysophospholipase D (LysoPLD). Species Sequence Homology: mouse; 100% (16/16aa), human and chimpanzee; 93.75% (15/16aa).
Species Sequence Homology
Mouse; 100% (16/16aa), human and chimpanzee; 93.75% (15/16aa).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot of human cerebella supernatant (40ug) using MBS611438.)
Western Blot (WB)
(Western Blot of human cerebella supernatant (40ug) using MBS611438.)
Immunofluorescence (IF)
(Immunofluorescent staining of HepG2 cells with MBS611438 (2ug/ml). The positive reaction is visualized by a Cy3-conjugated donkey anti-rabbit secondary antibody. (Note: the cytoplasmic and membrane localization of the protein).)
Immunofluorescence (IF)
(Immunofluorescent staining of HepG2 cells with MBS611438 (2ug/ml). The positive reaction is visualized by a Cy3-conjugated donkey anti-rabbit secondary antibody. (Note: the cytoplasmic and membrane localization of the protein).)
Related Product Information for anti-Lysophospholipase D antibody
Lysophosphatidic acid (LPA) is an extracellular signaling lipid that evokes multiple biological functions including induction of platelet aggregation, smooth muscle contraction, and stimulation of cell proliferation and chemotaxis. Lysophospholipase D (LysoPLD) was first discovered in 1999 as the enzyme responsible for generating LPA from lysophosphatidylcholine (LPC). It was later revealed to be identical to an autocrine motility factor, autotaxin (ATX), which plays a role in tumor progression and metastasis. LysoPLD/ATX mRNA is widely expressed with highest levels found in brain, ovary, lung, intestine, and testis. Rat LysoPLD is composed of 885 amino acids with an estimated molecular weight of 101kD. The protein is reported to be heavily glycosylated and thus its apparent size on SDS-PAGE may be run as high as 125kD. Useful positive controls include cerebrospinal fluid, mouse ascites or seminal plasma.
Product Categories/Family for anti-Lysophospholipase D antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
lysophospholipase
Similar Products
Product Notes
The Lysophospholipase D (Catalog #AAA611438) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Lysophospholipase D (LysoPLD) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Lysophospholipase D can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot and Immunofluorescence. Dilution: Western Blot: 5ug/ml Immunofluorescence (IF-IC): 2ug/ml. Researchers should empirically determine the suitability of the Lysophospholipase D for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lysophospholipase D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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