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Western Blot (WB) (Western blot analysis of LYRM3 in human liver tissue lysate with LYRM3 antibody at (A) 1 and (B) 2 μg/mL.)

Rabbit LYRM3 Polyclonal Antibody | anti-NDUFB9 antibody

LYRM3 Antibody

Gene Names
NDUFB9; B22; LYRM3; CI-B22; UQOR22
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
LYRM3 Antibody is affinity chromatography purified via peptide column.
Synonyms
LYRM3; Polyclonal Antibody; LYRM3 Antibody; B22; CI-B22; UQOR22; Complex I-B22; NADH dehydrogenase (ubiquinone) 1 beta subcomplex; 9; 22kDa; anti-NDUFB9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
LYRM3 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
179
Applicable Applications for anti-NDUFB9 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
LYRM3 antibody can be used for detection of LYRM3 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
LYRM3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human LYRM3.
Buffer
LYRM3 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
LYRM3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of LYRM3 in human liver tissue lysate with LYRM3 antibody at (A) 1 and (B) 2 μg/mL.)

Western Blot (WB) (Western blot analysis of LYRM3 in human liver tissue lysate with LYRM3 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of LYRM3 in rat liver tissue with LYRM3 antibody at 5 μg/mL.)

Immunohistochemistry (IHC) (Immunohistochemistry of LYRM3 in rat liver tissue with LYRM3 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of LYRM3 in rat liver tissue with LYRM3 antibody at 20 μg/mL.)

Immunofluorescence (IF) (Immunofluorescence of LYRM3 in rat liver tissue with LYRM3 antibody at 20 μg/mL.)
Related Product Information for anti-NDUFB9 antibody
LYRM3 Antibody: LYRM3, also known as NADH dehydrogenase (ubiquinone) 1 beta subcomplex 9 (NDUFB9), is a ubiquitously expressed LYR-motif containing protein. It has been suggested to be a candidate gene for the branchio-oto-renal (BOR) syndrome, which is characterized by branchial and renal abnormalities and heredity deafness disorders. Other than its LYR-motif, LYRM3 appears to have no functional or structural relationship to either LYRM1 or LYRM2.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
21,831 Da
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa
NCBI Official Symbol
NDUFB9
NCBI Official Synonym Symbols
B22; LYRM3; CI-B22; UQOR22
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; complex I-B22; complex I B22 subunit; LYR motif-containing protein 3; NADH-ubiquinone oxidoreductase B22 subunit
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
Protein Family
UniProt Gene Name
NDUFB9
UniProt Synonym Gene Names
LYRM3; UQOR22; CI-B22
UniProt Entry Name
NDUB9_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

NDUFB9: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I LYR family.

Protein type: Mitochondrial; EC 1.6.5.3; Oxidoreductase; Energy Metabolism - oxidative phosphorylation; EC 1.6.99.3

Chromosomal Location of Human Ortholog: 8q13.3

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; sensory perception of sound; mitochondrial electron transport, NADH to ubiquinone

Disease: Mitochondrial Complex I Deficiency

Research Articles on NDUFB9

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Product Notes

The NDUFB9 ndufb9 (Catalog #AAA150080) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LYRM3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LYRM3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). LYRM3 antibody can be used for detection of LYRM3 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the NDUFB9 ndufb9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LYRM3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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