Western Blot (WB)
(Western Blot analysis of PLA2G15 expression in transfected 293T cell line by PLA2G15 polyclonal antibody. Lane 1: LYPLA3 transfected lysate (46.7kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human LYPLA3 Polyclonal Antibody | anti-LYPLA3 antibody
LYPLA3 (PLA2G15, Group XV Phospholipase A2, 1-O-acylceramide Synthase, ACS, LCAT-like Lysophospholipase, LLPL, Lysophospholipase 3, Lysosomal Phospholipase A2, LPLA2, UNQ341/PRO540) (AP)
Gene Names
PLA2G15; ACS; LLPL; LPLA2; LYPLA3; GXVPLA2
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
LYPLA3; Polyclonal Antibody; LYPLA3 (PLA2G15; Group XV Phospholipase A2; 1-O-acylceramide Synthase; ACS; LCAT-like Lysophospholipase; LLPL; Lysophospholipase 3; Lysosomal Phospholipase A2; LPLA2; UNQ341/PRO540) (AP); anti-LYPLA3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human LYPLA3.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-LYPLA3 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human LYPLA3, aa1-412 (NP_036452.1).
Immunogen Sequence
MGLHLRPYRVGLLPDGLLFLLLLLMLLADPALPAGRHPPVVLVPGDLGNQLEAKLDKPTVVHYLCSKKTESYFTIWLNLELLLPVIIDCWIDNIRLVYNKTSRATQFPDGVDVRVPGFGKTFSLEFLDPSKSSVGSYFHTMVESLVGWGYTRGEDVRGAPYDWRRAPNENGPYFLALREMIEEMYQLYGGPVVLVAHSMGNMYTLYFLQRQPQAWKDKYIRAFVSLGAPWGGVAKTLRVLASGDNNRIPVIGPLKIREQQRSAVSTSWLLPYNYTWSPEKVFVQTPTINYTLRDYRKFFQDIGFEDGWLMRQDTEGLVEATMPPGVQLHCLYGTGVPTPDSFYYESFPDRDPKICFGDGDGTVNLKSALQCQAWQSRQEHQVLLQELPGSEHIEMLANATTLAYLKRVLLGP
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of PLA2G15 expression in transfected 293T cell line by PLA2G15 polyclonal antibody. Lane 1: LYPLA3 transfected lysate (46.7kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of PLA2G15 expression in transfected 293T cell line by PLA2G15 polyclonal antibody. Lane 1: LYPLA3 transfected lysate (46.7kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-LYPLA3 antibody
Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities.
Product Categories/Family for anti-LYPLA3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,658 Da
NCBI Official Full Name
group XV phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2, group XV
NCBI Official Symbol
PLA2G15
NCBI Official Synonym Symbols
ACS; LLPL; LPLA2; LYPLA3; GXVPLA2
NCBI Protein Information
group XV phospholipase A2; 1-O-acylceramide synthase; lysosomal phospholipase A2; LCAT-like lysophospholipase; lysophospholipase 3 (lysosomal phospholipase A2)
UniProt Protein Name
Group XV phospholipase A2
UniProt Gene Name
PLA2G15
UniProt Synonym Gene Names
LYPLA3; ACS; LLPL1 PublicationManual assertion based on opinion iniRef.1; LPLA22 PublicationsManual assertion based on opinion iniRef.11
Uniprot Description
Has transacylase and calcium-independent phospholipase A2 activity (PubMed:20410020, PubMed:23958596). Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid (PubMed:11790796, PubMed:25727495). Has high activity with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), catalyzing the transfer of oleic acid to N-acetyl-sphingosine. Required for normal phospholipid degradation in alveolar and peritoneal macrophages and in spleen (). May have weak lysophospholipase activity (PubMed:10092508).
Similar Products
Product Notes
The LYPLA3 pla2g15 (Catalog #AAA6384521) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LYPLA3 (PLA2G15, Group XV Phospholipase A2, 1-O-acylceramide Synthase, ACS, LCAT-like Lysophospholipase, LLPL, Lysophospholipase 3, Lysosomal Phospholipase A2, LPLA2, UNQ341/PRO540) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LYPLA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the LYPLA3 pla2g15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LYPLA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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