Rabbit anti-Human, Mouse LYPL1 Polyclonal Antibody | anti-LYPL1 antibody

LYPL1, ID (LYPLAL1, Lysophospholipase-like protein 1) (AP)

Cat. Num. AAA6317703

• Gene Names: LYPLAL1; Q96AV0
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LYPL1; Polyclonal Antibody; ID (LYPLAL1; Lysophospholipase-like protein 1) (AP); LYPLAL1; Lysophospholipase-like protein 1; anti-LYPL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-LYPL1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: LYPL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-127 amino acids from the Central region of human LYPL1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(LYPL1 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LYPL1 antibody detected the LYPL1 protein (arrow).)

Immunohistochemistry (IHC)

(LYPL1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LYPL1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(LYPL1 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-LYPL1 antibody

The exact function of this protein remains unknown.

Product Categories/Family for anti-LYPL1 antibody

Antibodies; Enzymes

References

Hotta, K., et al. J. Hum. Genet. (2010). In press: Lindgren, C.M., et al. PLoS Genet. 5 (6), E1000508 (2009): David, D., et al. Genomics 81(5):489-503(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 20270341
• NCBI GeneID: 127018
• NCBI Accession #: NP_620149.1
• NCBI GenBank Nucleotide #: NM_138794.3
• UniProt Accession #: Q5VWZ2; Q5VWZ3; Q7Z4A3; Q96AV0; A8K677
• Molecular Weight: 24,476 Da
• NCBI Official Full Name: lysophospholipase-like protein 1
• NCBI Official Synonym Full Names: lysophospholipase like 1
• NCBI Official Symbol: LYPLAL1
• NCBI Official Synonym Symbols: Q96AV0
• NCBI Protein Information: lysophospholipase-like protein 1
• UniProt Protein Name: Lysophospholipase-like protein 1
• UniProt Gene Name: LYPLAL1
• UniProt Entry Name: LYPL1_HUMAN

Uniprot Description

LYPLAL1: Does not exhibit phospholipase nor triacylglycerol lipase activity, able to hydrolyze only short chain substrates due to its shallow active site. Belongs to the AB hydrolase 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.2.-; Phospholipase

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: cytoplasm; cytosol

Molecular Function: lysophospholipase activity

Biological Process: negative regulation of Golgi to plasma membrane protein transport; protein depalmitoylation