Rabbit anti-Human LUC7L2 Polyclonal Antibody | anti-LUC7L2 antibody

LUC7L2, CT (LUC7L2, Putative RNA-binding protein Luc7-like 2) (PE)

Cat. Num. AAA6317581

• Gene Names: LUC7L2; CGI-59; CGI-74; LUC7B2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LUC7L2; Polyclonal Antibody; CT (LUC7L2; Putative RNA-binding protein Luc7-like 2) (PE); Putative RNA-binding protein Luc7-like 2; anti-LUC7L2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-LUC7L2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: LUC7L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348~377 amino acids from the C-terminal region of human LUC7L2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of LUC7L2 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). LUC7L2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(LUC7L2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LUC7L2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(LUC7L2 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-LUC7L2 antibody

LUC7L2 may bind to RNA via its Arg/Ser-rich domain.

Product Categories/Family for anti-LUC7L2 antibody

Antibodies; RNA Binding Proteins

References

Webby, C.J., et al. Science 325(5936):90-93(2009). Howell, V.M., et al. Hum. Mol. Genet. 16(20):2506-2516(2007). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Puig, O., et al. Nucleic Acids Res. 35(17):5874-5885(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 347446678
• NCBI GeneID: 51631
• NCBI Accession #: NP_001231514.1
• NCBI GenBank Nucleotide #: NM_001244585.1
• UniProt Accession #: Q9Y383; Q8IUP9; Q9NVL3; Q9NVN7; Q9UQN1; B7Z500
• Molecular Weight: 46,514 Da
• NCBI Official Full Name: putative RNA-binding protein Luc7-like 2 isoform 3
• NCBI Official Synonym Full Names: LUC7-like 2 (S. cerevisiae)
• NCBI Official Symbol: LUC7L2
• NCBI Official Synonym Symbols: CGI-59; CGI-74; LUC7B2
• NCBI Protein Information: putative RNA-binding protein Luc7-like 2
• UniProt Protein Name: Putative RNA-binding protein Luc7-like 2
• Protein Family: Putative RNA-binding protein
• UniProt Gene Name: LUC7L2
• UniProt Entry Name: LC7L2_HUMAN

NCBI Description

This gene encodes a protein that contains a C2H2-type zinc finger, coiled-coil region and arginine, serine-rich (RS) domain. A similar protein in mouse interacts with sodium channel modifier 1, and the encoded protein may be involved in the recognition of non-consensus splice donor sites in association with the U1 snRNP spliceosomal subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

LUC7L2: a Luc7 family protein that may bind RNA via its Arg/Ser-rich domain. Contains 1 C2H2-type zinc finger. Two alternatively spliced isoforms have been described.

Protein type: C2H2-type zinc finger protein; RNA-binding

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: nuclear speck; snRNP U1

Molecular Function: mRNA binding; protein binding; enzyme binding

Biological Process: mRNA splice site selection