Western Blot (WB)
(Western blot analysis of LTF using LOVO whole cell lysates)
Rabbit anti-Human LTF Polyclonal Antibody | anti-LTF antibody
LTF Antibody
Gene Names
LTF; LF; HLF2; GIG12; HEL110
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
LTF; Polyclonal Antibody; LTF Antibody; GIG12; Growth inhibiting protein 12; HLF2; Kaliocin 1; Lactoferrin; Lactoferroxin-C; Lactotransferrin; LF; Neutrophil lactoferrin; Talalactoferrin; TRFL_HUMAN; anti-LTF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
LTF antibody detects endogenous levels of total LTF
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
666
Applicable Applications for anti-LTF antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human LTF
Subcellular Location
Secreted.
Tissue Specificity
High levels are found in saliva and tears, intermediate levels in serum and plasma, and low levels in urine. In kidney, detected in the distal collecting tubules in the medulla but not in the cortical region or in blood vessels. Detected in peripheral blood neutrophils (at protein level). Isoform 1 and isoform DeltaLf are expressed in breast, prostate, spleen, pancreas, kidney, small intestine, lung, skeletal muscle, uterus, thymus and fetal liver. Isoform 1 is expressed in brain, testis and peripheral blood leukocytes; isoform DeltaLf is barely detectable in these tissues. Isoform DeltaLf is expressed in placenta, liver and ovary; isoform 1 is barely detectable in these tissues. In kidney, isoform 1 is expressed at high levels in the collecting tubules of the medulla but at very low levels in the cortex.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit
Similarity
Bovine (88%), Horse (100%), Sheep (88%), Rabbit (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of LTF using LOVO whole cell lysates)
Western Blot (WB)
(Western blot analysis of LTF using LOVO whole cell lysates)
Related Product Information for anti-LTF antibody
Function: Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate.
Subunit Structure: Monomer. Found in a complex with LTF, CLU, EPPIN and SEMG1.
Post-translational Modifications: Isoform DeltaLf: Ubiquitinated at Lys-379 and Lys-391. Poly-N-acetyllactosaminic carbohydrate moiety seems to be needed for TLR4 activation.
Similarity: Belongs to the transferrin family.
Subunit Structure: Monomer. Found in a complex with LTF, CLU, EPPIN and SEMG1.
Post-translational Modifications: Isoform DeltaLf: Ubiquitinated at Lys-379 and Lys-391. Poly-N-acetyllactosaminic carbohydrate moiety seems to be needed for TLR4 activation.
Similarity: Belongs to the transferrin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 78 kDa
Predicted: 79 kDa
Predicted: 79 kDa
NCBI Official Full Name
lactotransferrin isoform 2
NCBI Official Synonym Full Names
lactotransferrin
NCBI Official Symbol
LTF
NCBI Official Synonym Symbols
LF; HLF2; GIG12; HEL110
NCBI Protein Information
lactotransferrin
UniProt Protein Name
Lactotransferrin
Protein Family
UniProt Gene Name
LTF
UniProt Synonym Gene Names
GIG12; LF; Lactoferrin; Lfcin-H
NCBI Description
This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
Uniprot Description
Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate.
Research Articles on LTF
Similar Products
Product Notes
The LTF ltf (Catalog #AAA9608526) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LTF Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LTF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the LTF ltf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LTF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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