LTB4R2 / BLT2 Polyclonal Antibody | anti-LTB4R2 antibody

Rabbit Polyclonal to Human LTB4R2 / BLT2

Cat. Num. AAA247744

• Gene Names: LTB4R2; BLT2; NOP9; BLTR2; JULF2; KPG_004; LTB4-R2; LTB4-R 2
• Reactivity: Human ; Predicted Reactivity: Monkey, Hamster
• Applications: Immunohistochemistry
• Purity: Immunoaffinity purified
• Synonyms: LTB4R2 / BLT2; Polyclonal Antibody; Rabbit Polyclonal to Human LTB4R2 / BLT2; Anti-LTB4R2 / BLT2 Antibody (C-Terminus) IHC-plus; LTB4R2; BLT2; BLTR2; Blt-2; BLT2R; JULF2; LTB4-R2; Hblt2; KPG_004; Leukotriene B4 receptor 2; Leukotriene B4 receptor BLT2; LTB4 receptor JULF2; LTB4-R 2; Human LTB4R2; anti-LTB4R2 antibody

• Host: LTB4R2 / BLT2 antibody was produced in Rabbit
• Reactivity: Human ; Predicted Reactivity: Monkey, Hamster
• Clonality: Polyclonal
• Specificity: Human LTB4R2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, 0.1% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-LTB4R2 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (4 ug/ml); (Optimal dilution to be determined by the researcher)
• Target: LTB4R2 / BLT2
• Family: GPCR / Chemoattract
• Immunogen Species: LTB4R2 / BLT antibody was raised against Human
• Antigen Type: Synthetic peptide
• Immunogen: Synthetic 17 amino acid peptide from C-Terminus of human LTB4R2. Percent identity with other species by BLAST analysis: Human, Gorilla, Marmoset (100%); Monkey, Hamster (94%); Mouse, Rat (88%); Panda (82%).
• Epitope: C-Terminus
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT
• Preparation and Storage: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-LTB4R2 / BLT2 antibody IHC of human Pancreas, Carcinoma and Leukocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-LTB4R2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-LTB4R2 / BLT2 antibody IHC of human Lymph Node, Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-LTB4R2 antibody

BLTR2 is a Chemoattractant Receptor that causes LTB4-induced chemotaxis, calcium mobilization, and inhibition of adenylyl cyclase. Portions of the coding regions of BLTR2 have been duplicated and are part of the 5' UTR noncoding regions of the clusters LS190955 (BLT1) and LS54843 (CIDE-B) on chromosome 14.

Product Categories/Family for anti-LTB4R2 antibody

Family: GPCR; Subfamily: Chemoattractant

NCBI and Uniprot Product Information

• NCBI GI #: 291042665
• NCBI GeneID: 56413
• NCBI Accession #: NP_062813.2
• NCBI GenBank Nucleotide #: NM_019839.4
• UniProt Accession #: Q9NPC1; Q5KU28; Q9NPE5
• NCBI Official Full Name: leukotriene B4 receptor 2
• NCBI Official Synonym Full Names: leukotriene B4 receptor 2
• NCBI Official Symbol: LTB4R2
• NCBI Official Synonym Symbols: BLT2; NOP9; BLTR2; JULF2; KPG_004; LTB4-R2; LTB4-R 2
• NCBI Protein Information: leukotriene B4 receptor 2; LTB4 receptor JULF2; leukotriene B4 receptor BLT2; seven transmembrane receptor BLTR2
• UniProt Protein Name: Leukotriene B4 receptor 2
• Protein Family: Leukotriene B4 receptor
• UniProt Gene Name: LTB4R2
• UniProt Synonym Gene Names: BLT2R; BLTR2; LTB4-R 2; LTB4-R2
• UniProt Entry Name: LT4R2_HUMAN

Uniprot Description

LTB4R2: Low-affinity receptor for leukotrienes including leukotriene B4. Mediates chemotaxis of granulocytes and macrophages. The response is mediated via G-proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinities for the leukotrienes is LTB4 > 12-epi-LTB4 > LTB5 > LTB3. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Motility/polarity/chemotaxis; Receptor, GPCR; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: leukotriene B4 receptor activity; leukotriene receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of adenylate cyclase activity; chemotaxis; keratinocyte migration