Rabbit anti-Human, Mouse LSM2 Polyclonal Antibody | anti-LSM2 antibody

LSM2 Antibody (C-Term)

Cat. Num. AAA9216621

• Gene Names: LSM2; G7B; snRNP; C6orf28; YBL026W
• Reactivity: Human, Mouse
• Applications: Western Blot
• Synonyms: LSM2; Polyclonal Antibody; LSM2 Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); U6 snRNA-associated Sm-like protein LSm2; Protein G7b; Small nuclear ribonuclear protein D homolog; snRNP core Sm-like protein Sm-x5; C6orf28; G7B; anti-LSM2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This LSM2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 55-89 amino acids from human LSM2.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 95

• Applicable Applications for anti-LSM2 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-LSM2 Antibody (C-Term) at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: Hela whole cell lysateLane 3: K562 whole cell lysateLane 4: MCF-7 whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 11 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-LSM2 antibody

Binds specifically to the 3'-terminal U-tract of U6 snRNA. May be involved in pre-mRNA splicing.

Product Categories/Family for anti-LSM2 antibody

Crown Antibodies

References

Achsel T.,et al.EMBO J. 18:5789-5802(1999). Olavesen M.G.,et al.Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases. Schmarda A.,et al.Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases. Xie T.,et al.Genome Res. 13:2621-2636(2003). Shiina S.,et al.Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 10863977
• NCBI GeneID: 57819
• NCBI Accession #: NP_067000.1
• NCBI GenBank Nucleotide #: NM_021177.4
• UniProt Accession #: Q9Y333; Q6FGG1
• Molecular Weight: 10835
• NCBI Official Full Name: U6 snRNA-associated Sm-like protein LSm2
• NCBI Official Synonym Full Names: LSM2 homolog, U6 small nuclear RNA and mRNA degradation associated
• NCBI Official Symbol: LSM2
• NCBI Official Synonym Symbols: G7B; snRNP; C6orf28; YBL026W
• NCBI Protein Information: U6 snRNA-associated Sm-like protein LSm2
• UniProt Protein Name: U6 snRNA-associated Sm-like protein LSm2
• Protein Family: Sm-like protein
• UniProt Gene Name: LSM2
• UniProt Synonym Gene Names: C6orf28; G7B
• UniProt Entry Name: LSM2_HUMAN

NCBI Description

This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 19. [provided by RefSeq, Nov 2011]

Uniprot Description

LSM2: Binds specifically to the 3'-terminal U-tract of U6 snRNA. May be involved in pre-mRNA splicing. Identified in the spliceosome C complex. LSm subunits form a heteromer with a doughnut shape. Belongs to the snRNP Sm proteins family.

Protein type: RNA splicing; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; U4/U6 x U5 tri-snRNP complex; cytosol

Molecular Function: protein binding; Ral GTPase binding; protein kinase binding; U6 snRNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA catabolic process, deadenylation-dependent decay