Rabbit anti-Human LRTOMT Polyclonal Antibody | anti-LRTOMT antibody

LRTOMT (Transmembrane O-Methyltransferase, Catechol O-Methyltransferase 2, Protein LRTOMT2, LRTOMT, COMT2, TOMT) (MaxLight 650)

Cat. Num. AAA6457094

• Gene Names: LRTOMT; DFNB63; LRRC51; CFAP111
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LRTOMT; Polyclonal Antibody; LRTOMT (Transmembrane O-Methyltransferase; Catechol O-Methyltransferase 2; Protein LRTOMT2; COMT2; TOMT) (MaxLight 650); anti-LRTOMT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LRTOMT. Species Crossreactivity: human
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-LRTOMT antibody: Western Blot (WB)
• Application Notes: WB: 1:4000; Applications are based on unconjugated antibody.
• Immunogen: KLH conjugated synthetic peptide corresponding to aa2-36 at the N-terminal region of human LRTOMT.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-LRTOMT antibody

Antibodies; Transmembrane Proteins

References

Ahmed Z.M., et al. Nat. Genet. 40:1335-1340(2008). Wan D., et al. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004). Ota T., et al. Nat. Genet. 36:40-45(2004). Taylor T.D., et al. Nature 440:497-500(2006). Du X., et al. Proc. Natl. Acad. Sci. U.S.A. 105:14609-14614(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 223718159
• NCBI GeneID: 220074
• NCBI Accession #: NP_001138780.1
• NCBI GenBank Nucleotide #: NM_001145308.4
• UniProt Accession #: Q8WZ04; B7Z816; Q96E66
• Molecular Weight: 27,548 Da
• NCBI Official Full Name: transmembrane O-methyltransferase isoform LRTOMT2a
• NCBI Official Synonym Full Names: leucine rich transmembrane and O-methyltransferase domain containing
• NCBI Official Symbol: LRTOMT
• NCBI Official Synonym Symbols: DFNB63; LRRC51; CFAP111
• NCBI Protein Information: leucine-rich repeat-containing protein 51; transmembrane O-methyltransferase
• UniProt Protein Name: Transmembrane O-methyltransferase
• UniProt Gene Name: LRTOMT
• UniProt Entry Name: TOMT_HUMAN

NCBI Description

This gene includes two transcript forms. The short form has one open reading frame (ORF), which encodes the leucine-rich repeats (LRR)-containing protein of unknown function. This protein is called LRTOMT1 or LRRC51. The long form has two alternative ORFs; the upstream ORF has the same translation start codon as used in the short form and the resulting transcript is a candidate for nonsense-mediated decay, and the downstream ORF encodes a different protein, which is a transmembrane catechol-O-methyltransferase and is called LRTOMT2, TOMT or COMT2. The COMT2 is essential for auditory and vestibular function. Defects in the COMT2 can cause nonsyndromic deafness. Alternatively spliced transcript variants from each transcript form have been found for this gene. [provided by RefSeq, Sep 2012]

Uniprot Description

LRTOMT: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Required for auditory function. Defects in LRTOMT are the cause of deafness autosomal recessive type 63 (DFNB63). A form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the methyltransferase superfamily. Catechol-O-methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; Cell development/differentiation; Membrane protein, integral; EC 2.1.1.6

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: cytoplasm; integral to membrane

Molecular Function: catechol O-methyltransferase activity

Biological Process: auditory receptor cell development; catecholamine catabolic process; methylation; neurotransmitter catabolic process; sensory perception of sound

Disease: Deafness, Autosomal Recessive 63