Rabbit LRP5 Polyclonal Antibody | anti-LRP5 antibody

LRP5 Polyclonal Antibody

Cat. Num. AAA126119

• Gene Names: LRP5; HBM; LR3; OPS; EVR1; EVR4; LRP7; OPPG; BMND1; OPTA1; VBCH2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Affinity purification
• Synonyms: LRP5; Polyclonal Antibody; LRP5 Polyclonal Antibody; LRP5 ; BMND1; EVR1; EVR4; HBM; LR3; LRP7; OPPG; OPS; OPTA1; VBCH2;; anti-LRP5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 1615

• Applicable Applications for anti-LRP5 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: Western Blot (WB): 1:200-1: 500; Immunohistochemistry (IHC): 1:50-1: 100; Immunoprecipitation (IP): 1:20-1: 50; Immunofluorescence (IF): 1:20-1: 50
• Immunogen: A synthetic -peptide of human LRP5
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using LRP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver using LRP5 antibody at dilution of 1:200 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate using LRP5 antibody at dilution of 1:200 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse lung using LRP5 antibody at dilution of 1:200 (40x lens).)

Related Product Information for anti-LRP5 antibody

LRP5 and LRP6 are singlepass transmembrane proteins belonging to the lowdensity lipoprotein receptor (LDLR)related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain, and prolinerich motifs in the cytoplasmic domain (1). They function as coreceptors for Wnt and are required for the canonical Wnt/betacatenin signaling pathway (2,3). LRP5 and LRP6 are highly homologous and have redundant roles during development (4,5). The activity of LRP5 and LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins (6,7). Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490, and Thr1493 by kinases such as GSK-3 and CK1 (8-10). Phosphorylated LRP6 recruits axin to the membrane and presumably activates betacatenin signaling (8-10). LRP5 is involved in the regulation of bone homeostasis. Mutations and polymorphisms in LPR5 are associated with bone diseases like osteoporosispseudoglioma syndrome and highbonemass disorders (11-13). In addition, mutations in LRP5 are found in patients with hyperparathyroid tumor and breast cancer (14,15).

References

1. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248, 879-888. 2. Pinson, K.I. et al. (2000) Nature 407, 535-538. 3. Tamai, K. et al. (2000) Nature 407, 530-535. 4. Kelly, O.G. et al. (2004) Development 131, 2803-2815. 5. He, X. et al. (2004) Development 131, 1663-1677. 6. Semënov, M.V. et al. (2001) Curr Biol 11, 951-61. 7. Bafico, A. et al. (2001) Nat. Cell Biol. 3, 683-668. 8. Tamai, K. et al. (2004) Mol. Cell 13, 149-156. 9. Zeng, X. et al. (2005) Nature 438, 873-877. 10. Davidson, G. et al. (2005) Nature 438, 867-872. 11. Levasseur, R. et al. (2005) Joint Bone Spine 72, 207-14. 12. Ferrari, S.L. et al. (2005) Curr Opin Lipidol 16, 207-14. 13. Balemans, W. and Van Hul, W. (2007) Endocrinology 148, 2622-9. 14. Björklund, P. et al. (2007) PLoS Med 4, e328. 15. Björklund, P. et al. (2009) PLoS One 4, e4243.

NCBI and Uniprot Product Information

• NCBI GI #: 119709832
• NCBI GeneID: 4041
• NCBI Accession #: NP_002326.2
• NCBI GenBank Nucleotide #: NM_002335.2
• UniProt Accession #: O75197; Q96TD6; Q9UES7; Q9UP66
• Molecular Weight: 179kDa
• NCBI Official Full Name: low-density lipoprotein receptor-related protein 5
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein 5
• NCBI Official Symbol: LRP5
• NCBI Official Synonym Symbols: HBM; LR3; OPS; EVR1; EVR4; LRP7; OPPG; BMND1; OPTA1; VBCH2
• NCBI Protein Information: low-density lipoprotein receptor-related protein 5; LRP-5; low density lipoprotein receptor-related protein 7
• UniProt Protein Name: Low-density lipoprotein receptor-related protein 5
• Protein Family: Low-density lipoprotein receptor-related protein
• UniProt Gene Name: LRP5
• UniProt Synonym Gene Names: LR3; LRP7; LRP-5
• UniProt Entry Name: LRP5_HUMAN