Goat anti-Human LRP4 Polyclonal Antibody | anti-LRP4 antibody

LRP4, ID (Low Density Lipoprotein Receptor-related Protein 4, LRP-4, Multiple Epidermal Growth Factor-like Domains 7, KIAA0816, LRP10, MEGF7)

Cat. Num. AAA6007623

• Gene Names: LRP4; CLSS; LRP-4; LRP10; MEGF7; SOST2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: LRP4; Polyclonal Antibody; ID (Low Density Lipoprotein Receptor-related Protein 4; LRP-4; Multiple Epidermal Growth Factor-like Domains 7; KIAA0816; LRP10; MEGF7); Anti -LRP4; anti-LRP4 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human LRP4. Species sequence homology: bovine, rat and mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-LRP4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:32,000; Western Blot: 0.1-0.3ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/ml
• Immunogen: Synthetic peptide C-SNPSYRTSTQEVK from an internal region of human LRP4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Colon, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE))

Chemiluminescence

(LRP4 antibody (0.1ug/ml) staining of human cerebral cortex lysate (35ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-LRP4 antibody

Low-density lipoprotein receptor-related protein 4 (LRP-4) is a novel, single-pass type I membrane protein belonging to the LDLR family and is comprised of three EGF-like domains, eight LDL-receptor class A domains, and twenty LDL-receptor class B repeats. The exact function of LRP-4 has yet to be elucidated, however it is thought to function as a cell surface receptor with the ability to both bind and neutralize extracellular ligands, internalizing them for lysosomal degradation. Expression has been observed in various regions of the brain.

Product Categories/Family for anti-LRP4 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 157384998
• NCBI GeneID: 4038
• NCBI Accession #: NP_002325.2
• NCBI GenBank Nucleotide #: NM_002334.3
• UniProt Accession #: O75096; Q4AC85; Q5KTZ5; B2RN39
• Molecular Weight: 212,045 Da
• NCBI Official Full Name: low-density lipoprotein receptor-related protein 4
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein 4
• NCBI Official Symbol: LRP4
• NCBI Official Synonym Symbols: CLSS; LRP-4; LRP10; MEGF7; SOST2
• NCBI Protein Information: low-density lipoprotein receptor-related protein 4; multiple epidermal growth factor-like domains 7
• UniProt Protein Name: Low-density lipoprotein receptor-related protein 4
• Protein Family: Low-density lipoprotein receptor-related protein
• UniProt Gene Name: LRP4
• UniProt Synonym Gene Names: KIAA0816; LRP10; MEGF7; LRP-4
• UniProt Entry Name: LRP4_HUMAN

NCBI Description

This gene encodes a member of the low-density lipoprotein receptor-related protein family. The encoded protein may be a regulator of Wnt signaling. Mutations in this gene are associated with Cenani-Lenz syndrome. [provided by RefSeq, May 2010]

Uniprot Description

LRP4: Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN- induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. Defects in LRP4 are the cause of Cenani-Lenz syndactyly syndrome (CLSS). It is a congenital malformation syndrome defined as complete and complex syndactyly of the hands combined with malformations of the forearm bones and similar manifestations in the lower limbs. Defects in LRP4 are the cause of sclerosteosis type 2 (SOST2). A sclerosing bone dysplasia characterized by a generalized hyperostosis and sclerosis leading to a markedly thickened skull, with mandible, ribs, clavicles and all long bones also being affected. Due to narrowing of the foramina of the cranial nerves, facial nerve palsy, hearing loss and atrophy of the optic nerves can occur. Sclerosteosis is clinically and radiologically very similar to van Buchem disease, mainly differentiated by hand malformations and a large stature in sclerosteosis patients. Belongs to the LDLR family.

Protein type: Cell surface; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cell surface; cell soma; postsynaptic density; dendrite; integral to membrane; flotillin complex; neuromuscular junction

Molecular Function: protein binding; protein homodimerization activity; apolipoprotein binding; calcium ion binding; receptor tyrosine kinase binding

Biological Process: limb development; regulation of protein amino acid phosphorylation; extracellular matrix organization and biogenesis; Wnt receptor signaling pathway; dendrite morphogenesis; endocytosis; odontogenesis of dentine-containing teeth; hair follicle development; dorsal/ventral pattern formation; synaptic growth at neuromuscular junction; synapse organization and biogenesis; negative regulation of ossification; protein heterotetramerization; negative regulation of axonogenesis; embryonic digit morphogenesis; kidney development; proximal/distal pattern formation

Disease: Myasthenic Syndrome, Congenital, 17; Cenani-lenz Syndactyly Syndrome; Sclerosteosis 2