Rabbit anti-Human LPIN2 Polyclonal Antibody | anti-LPIN2 antibody

LPIN2 Antibody (Center)

Cat. Num. AAA9212263

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot, Immunohistochemistry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: LPIN2; Polyclonal Antibody; LPIN2 Antibody (Center); Phosphatidate phosphatase LPIN2; Lipin-2; KIAA0249; anti-LPIN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This LPIN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262-288 amino acids from the Central region of human LPIN2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 262-288
• Sequence Length: 896

• Applicable Applications for anti-LPIN2 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of LPIN2 Antibody (Center) in K562 cell line lysates (35ug/lane).LPIN2 (arrow) was detected using the purified Pab.(2ug/ml))

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with LPIN2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of K562 cells using LPIN2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-LPIN2 antibody

Defects in LPIN2 are the cause of Majeed syndrome. Majeed syndrome is an autosomal recessive disorder combining features of chronic recurrent multifocal osteomyelitis, congenital dyserythropoietic anemia and inflammatory dermatosis.

Product Categories/Family for anti-LPIN2 antibody

Cardiovascular; Metabolism; Signal transduction

References

Olsen,J.V., et.al., Cell 127 (3), 635-648 (2006)
Ferguson,P.J., et.al., J. Med. Genet. 42 (7), 551-557 (2005)

NCBI and Uniprot Product Information

• NCBI GI #: 7662022
• NCBI GeneID: 9663
• NCBI Accession #: NP_055461.1
• NCBI GenBank Nucleotide #: NM_014646.2
• UniProt Accession #: Q92539; A7MD25; D3DUH3
• Molecular Weight: 99399
• NCBI Official Full Name: phosphatidate phosphatase LPIN2
• NCBI Official Synonym Full Names: lipin 2
• NCBI Official Symbol: LPIN2
• NCBI Protein Information: phosphatidate phosphatase LPIN2
• UniProt Protein Name: Phosphatidate phosphatase LPIN2
• Protein Family: Phosphatidate phosphatase
• UniProt Gene Name: LPIN2
• UniProt Synonym Gene Names: KIAA0249
• UniProt Entry Name: LPIN2_HUMAN

NCBI Description

Mouse studies suggest that this gene functions during normal adipose tissue development and may play a role in human triglyceride metabolism. This gene represents a candidate gene for human lipodystrophy, characterized by loss of body fat, fatty liver, hypertriglyceridemia, and insulin resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

LPIN2: Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism. Defects in LPIN2 are the cause of Majeed syndrome (MAJEEDS). An autosomal recessive syndrome characterized by chronic recurrent multifocal osteomyelitis that is of early onset with a lifelong course, congenital dyserythropoietic anemia that presents as hypochromic, microcytic anemia during the first year of life and ranges from mild to transfusion-dependent, and transient inflammatory dermatosis, often manifesting as Sweet syndrome (neutrophilic skin infiltration). Belongs to the lipin family.

Protein type: Transcription, coactivator/corepressor; EC 3.1.3.4; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 18p11.31

Cellular Component: endoplasmic reticulum membrane; nucleus; cytosol

Molecular Function: phosphatidate phosphatase activity; transcription coactivator activity

Biological Process: transcription, DNA-dependent; dephosphorylation; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylethanolamine biosynthetic process; phosphatidylcholine biosynthetic process; triacylglycerol biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; lipid metabolic process; cellular lipid metabolic process; fatty acid catabolic process

Disease: Majeed Syndrome