Rabbit LOX Polyclonal Antibody | anti-LOX antibody

LOX Rabbit pAb

Cat. Num. AAA4754647

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: LOX; Polyclonal Antibody; LOX Rabbit pAb; lysyl oxidase; anti-LOX antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Heart, placenta, skeletal muscle, kidney, lung and pancreas.
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-LOX antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000; ELISA: 1:5000-1:10,000; IHC: 1:25-1:100
• Immunogen: Synthetic peptide of human LOX
• Conjugation: Unconjugated
• Molecular Function: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (PubMed:26838787). Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture.
• Modification: Unmodified
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Rat brain tissue lysate, Primary antibody: 160069(LOX Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 160069(LOX Antibody) at dilution 1/25.(Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 160069(LOX Antibody) at dilution 1/25. (Original magnification: ×200))

Related Product Information for anti-LOX antibody

This gene encodes a member of the lysyl oxidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a regulatory propeptide and the mature enzyme. The copper-dependent amine oxidase activity of this enzyme functions in the crosslinking of collagens and elastin, while the propeptide may play a role in tumor suppression.

Product Categories/Family for anti-LOX antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 257065
• NCBI GeneID: 4015
• UniProt Accession #: P28300; Q5FWF0; B2R5Q3
• Molecular Weight: 46,944 Da
• NCBI Official Full Name: lysyl oxidase
• NCBI Official Synonym Full Names: lysyl oxidase
• NCBI Official Symbol: LOX
• NCBI Protein Information: protein-lysine 6-oxidase
• UniProt Protein Name: Protein-lysine 6-oxidase
• Protein Family: Linoleate 9/13-lipoxygenase
• UniProt Gene Name: LOX
• UniProt Entry Name: LYOX_HUMAN

NCBI Description

The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]

Uniprot Description

LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.

Protein type: Secreted, signal peptide; EC 1.4.3.13; Secreted; Oxidoreductase

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: proteinaceous extracellular matrix; extracellular space; collagen; extracellular region; nucleus

Molecular Function: protein binding; copper ion binding; protein-lysine 6-oxidase activity

Biological Process: response to drug; elastic fiber assembly; blood vessel development; extracellular matrix organization and biogenesis; collagen fibril organization; wound healing; response to steroid hormone stimulus; protein modification process; lung development

Disease: Cutis Laxa, Autosomal Recessive, Type Ia