Western Blot (WB)
(Western BlotPositive WB detected in: MCF-7 whole cell lysate, HeLa whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate, Rat brain tissueAll lanes: LOX antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47 kDaObserved band size: 47 kDa)
Rabbit anti-Human, Rat LOX Polyclonal Antibody | anti-LOX antibody
LOX Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
IHC: 1:200-1:500
Western Blot (WB)
(Western BlotPositive WB detected in: MCF-7 whole cell lysate, HeLa whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate, Rat brain tissueAll lanes: LOX antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47 kDaObserved band size: 47 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: MCF-7 whole cell lysate, HeLa whole cell lysate, U87 whole cell lysate, U251 whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate, Rat brain tissueAll lanes: LOX antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47 kDaObserved band size: 47 kDa)
Immunohistochemistry (IHC)
(IHC image of MBS7133923 diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7133923 diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
Uniprot Description
LOX: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. Belongs to the lysyl oxidase family.
Protein type: Secreted, signal peptide; EC 1.4.3.13; Secreted; Oxidoreductase
Chromosomal Location of Human Ortholog: 5q23.2
Cellular Component: proteinaceous extracellular matrix; extracellular space; collagen; extracellular region; nucleus
Molecular Function: protein binding; copper ion binding; protein-lysine 6-oxidase activity
Biological Process: response to drug; elastic fiber assembly; blood vessel development; extracellular matrix organization and biogenesis; collagen fibril organization; wound healing; response to steroid hormone stimulus; protein modification process; lung development
Disease: Cutis Laxa, Autosomal Recessive, Type Ia
Research Articles on LOX
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Product Notes
The LOX lox (Catalog #AAA7133923) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LOX Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LOX can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000-1:5000 IHC: 1:200-1:500. Researchers should empirically determine the suitability of the LOX lox for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LOX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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