Rabbit anti-Human LONP1 Polyclonal Antibody | anti-LONP1 antibody

LONP1, ID (LONP1, PRSS15, Lon protease homolog, mitochondrial, LONHs, Lon protease-like protein, Mitochondrial ATP-dependent protease Lon, Serine protease 15) (Azide free) (HRP)

Cat. Num. AAA6316706

• Gene Names: LONP1; LON; LONP; PIM1; hLON; LonHS; PRSS15
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LONP1; Polyclonal Antibody; ID (LONP1; PRSS15; Lon protease homolog; mitochondrial; LONHs; Lon protease-like protein; Mitochondrial ATP-dependent protease Lon; Serine protease 15) (Azide free) (HRP); mitochondrial ATP-dependent protease Lon; Serine protease 15; anti-LONP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-LONP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: LONP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-418 amino acids from the Central region of human LONP1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LONP1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the LONP1 antibody detected the LONP1 protein (arrow).)

Related Product Information for anti-LONP1 antibody

This gene encodes a mitochondrial matrix protein in the Lon family of ATP-dependent proteases. A similar E. coli protein regulates gene expression by targeting specific regulatory proteins for degradation. This protein binds a specific sequence in the light and heavy chain promoters of the mitochondrial genome which are involved in regulation of DNA replication and transcription.

Product Categories/Family for anti-LONP1 antibody

Antibodies; Mitochondrial Proteins

References

Hansen, J., et al. Neuroscience 153(2):474-482(2008). Chen, S.H., et al. Nucleic Acids Res. 36(4):1273-1287(2008). Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008). Bayot, A., et al. Biochimie 90(2):260-269(2008). Xue, X., et al. Nan Fang Yi Ke Da Xue Xue Bao 27(6):870-874(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 21396489
• NCBI GeneID: 9361
• NCBI Accession #: NP_004784.2
• NCBI GenBank Nucleotide #: NM_004793.2
• UniProt Accession #: P36776; P36777; Q9UQ95; D6W635; E5KMH8
• Molecular Weight: 106,489 Da
• NCBI Official Full Name: lon protease homolog, mitochondrial
• NCBI Official Synonym Full Names: lon peptidase 1, mitochondrial
• NCBI Official Symbol: LONP1
• NCBI Official Synonym Symbols: LON; LONP; PIM1; hLON; LonHS; PRSS15
• NCBI Protein Information: lon protease homolog, mitochondrial; serine protease 15; protease, serine, 15; Lon protease-like protein; hLON ATP-dependent protease; mitochondrial lon peptidase 1; mitochondrial lon protease-like protein; mitochondrial ATP-dependent protease Lon
• UniProt Protein Name: Lon protease homolog, mitochondrial
• UniProt Gene Name: LONP1
• UniProt Entry Name: LONM_HUMAN

Uniprot Description

PRSS15: ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial promoters and RNA in a single- stranded, site-specific, and strand-specific manner. May regulate mitochondrial DNA replication and/or gene expression using site- specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters. Endogenous substrates include mitochondrial steroidogenic acute regulatory (StAR) protein. Belongs to the peptidase S16 family.

Protein type: RNA-binding; DNA-binding; Mitochondrial; EC 3.4.21.-; Protease

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: mitochondrion; membrane; mitochondrial matrix; cytoplasm

Molecular Function: G-quadruplex DNA binding; protein binding; single-stranded RNA binding; sequence-specific DNA binding; ATP-dependent peptidase activity; serine-type endopeptidase activity; ADP binding; ATP binding; single-stranded DNA binding

Biological Process: mitochondrion organization and biogenesis; mitochondrial genome maintenance; proteolysis involved in cellular protein catabolic process; misfolded or incompletely synthesized protein catabolic process; response to hypoxia; response to hormone stimulus; chaperone-mediated protein complex assembly; mitochondrial DNA metabolic process; protein homooligomerization; response to aluminum ion; aging

Disease: Codas Syndrome