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Immunohistochemistry (IHC) (Anti-LMX1B antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Rabbit anti-Human LMX1B Polyclonal Antibody | anti-LMX1B antibody

Rabbit Polyclonal to Human LMX1B

Gene Names
LMX1B; NPS1; LMX1.2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
LMX1B; Polyclonal Antibody; Rabbit Polyclonal to Human LMX1B; Anti-LMX1B Antibody (C-Terminus) IHC-plus; LMX1.2; NPS1; LIM/homeobox protein 1.2; LMX-1.2; LIM/homeobox protein LMX1B; Human LMX1B; anti-LMX1B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human LMX1B. At least three isoforms are known to exist. This antibody is predicted to not cross-react with LMX1A.
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.02% sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
402
Applicable Applications for anti-LMX1B antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes
IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Usage: LMX1B antibody can be used for detection of LMX1B by Western blot at 1-2 ug/mL.
Target Species
Human
Immunogen Description
A 17 amino acid peptide near the carboxy terminus of human LMX1B.
Immunogen Type
Synthetic peptide
Immunogen
LMX1B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human LMX1B.
Antigen Modification
C-Terminus
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-LMX1B antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-LMX1B antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Immunofluorescence of LMX1B in human brain tissue with LMX1B antibody at 20 ug/ml.)

Immunofluorescence (IF) (Immunofluorescence of LMX1B in human brain tissue with LMX1B antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot analysis of LMX1B in A-20 cell lysate with LMX1B antibody at (A) 1 and (B) 2 ug/ml.)

Western Blot (WB) (Western blot analysis of LMX1B in A-20 cell lysate with LMX1B antibody at (A) 1 and (B) 2 ug/ml.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,080 Da
NCBI Official Full Name
LIM homeobox transcription factor 1-beta isoform 2
NCBI Official Synonym Full Names
LIM homeobox transcription factor 1, beta
NCBI Official Symbol
LMX1B
NCBI Official Synonym Symbols
NPS1; LMX1.2
NCBI Protein Information
LIM homeobox transcription factor 1-beta; LMX-1.2; LIM/homeobox protein 1.2; LIM/homeobox protein LMX1B
UniProt Protein Name
LIM homeobox transcription factor 1-beta
Protein Family
UniProt Gene Name
LMX1B
UniProt Synonym Gene Names
LMX-1.2
UniProt Entry Name
LMX1B_HUMAN

NCBI Description

This gene encodes a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

LMX1B: Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels. Defects in LMX1B are the cause of nail-patella syndrome (NPS); also known as onychoosteodysplasia. NPS is a disease that cause abnormal skeletal patterning and renal dysplasia. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Cell development/differentiation; Transcription factor

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: nucleus

Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; transcription factor activity

Biological Process: collagen fibril organization; transcription, DNA-dependent; in utero embryonic development; multicellular organismal development; neuron migration; cerebellum morphogenesis; limb morphogenesis; neuron differentiation; organ growth; cell proliferation; regulation of transcription, DNA-dependent; dorsal/ventral pattern formation; midbrain development; positive regulation of transcription from RNA polymerase II promoter; central nervous system neuron development

Disease: Nail-patella Syndrome

Research Articles on LMX1B

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Product Notes

The LMX1B lmx1b (Catalog #AAA248062) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human LMX1B reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LMX1B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB). IHC-P (5 ug/ml), WB (1 - 2 ug/ml) Usage: LMX1B antibody can be used for detection of LMX1B by Western blot at 1-2 ug/mL. Researchers should empirically determine the suitability of the LMX1B lmx1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LMX1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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