Rabbit anti-Human, Mouse LMNA / Lamin A/C Polyclonal Antibody | anti-LMNA antibody

Rabbit Polyclonal (IgG) to Mouse LMNA / Lamin A/C

Cat. Num. AAA241812

• Gene Names: LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: LMNA / Lamin A/C; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Mouse LMNA / Lamin A/C; Anti-LMNA / Lamin A/C Antibody (C-Terminus) IHC-plus; LMNA; 70 kDa lamin; CDCD1; CMD1A; EMD2; FPLD; IDC; FPL; FPLD2; Lamin A/C; Lamin; LFP; LMNC; LMN1; LMNL1; HGPS; Prelamin-A/C; PRO1; Lamin A; Lamin A/C-like 1; LGMD1B; CDDC; CMT2B1; LDP1; Mouse LMNA; anti-LMNA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recombinant (partial), C-terminal. The immunogen is taken from isoform C and may not cross-react with isoform A.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
• Sequence Length: 572

• Applicable Applications for anti-LMNA antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF, IHC-P (1:50), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Mouse
• Immunogen Description: Recombinant mouse LMNA / Lamin A.
• Immunogen Type: Recombinant protein
• Immunogen: LMNA / Lamin A/C antibody was raised against recombinant mouse LMNA / Lamin A.
• Antigen Modification: C-Terminus
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-Lamin A/C antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

Western Blot (WB)

(Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal anti-lamin A. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.)

NCBI and Uniprot Product Information

• NCBI GI #: 5031875
• NCBI GeneID: 4000
• NCBI Accession #: NP_005563.1
• NCBI GenBank Nucleotide #: NM_005572.3
• UniProt Accession #: P02545; P02546; Q5I6Y4; Q5I6Y6; Q5TCJ2; Q5TCJ3; Q6UYC3; Q969I8; B4DI32; D3DVB0; D6RAQ3; E7EUI9
• Molecular Weight: 69,249 Da
• NCBI Official Full Name: lamin isoform C
• NCBI Official Synonym Full Names: lamin A/C
• NCBI Official Symbol: LMNA
• NCBI Official Synonym Symbols: FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
• NCBI Protein Information: lamin; 70 kDa lamin; prelamin-A/C; lamin A/C-like 1; renal carcinoma antigen NY-REN-32
• UniProt Protein Name: Prelamin-A/C
• Protein Family: Lamin
• UniProt Gene Name: LMNA
• UniProt Synonym Gene Names: LMN1
• UniProt Entry Name: LMNA_HUMAN

NCBI Description

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]

Uniprot Description

lamin A/C: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. Cleaved by caspase-6 during apoptosis into a 40-45 kDa and a28 kDa fragment.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: nucleoplasm; nuclear lamina; nuclear membrane; perinuclear region of cytoplasm; cytoplasm; lamin filament; intermediate filament; nuclear speck; nuclear envelope; cytosol; nucleus

Molecular Function: protein binding; structural molecule activity

Biological Process: muscle development; mitotic nuclear envelope reassembly; apoptosis; unfolded protein response; ventricular cardiac muscle cell development; mitotic nuclear envelope disassembly; regulation of cell migration; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; sterol regulatory element binding protein nuclear translocation; mitotic cell cycle; cell structure disassembly during apoptosis; establishment and/or maintenance of microtubule cytoskeleton polarity

Disease: Heart-hand Syndrome, Slovenian Type; Emery-dreifuss Muscular Dystrophy 2, Autosomal Dominant; Restrictive Dermopathy, Lethal; Muscular Dystrophy, Congenital, Lmna-related; Mandibuloacral Dysplasia With Type A Lipodystrophy; Charcot-marie-tooth Disease, Axonal, Type 2b1; Emery-dreifuss Muscular Dystrophy 3, Autosomal Recessive; Lipodystrophy, Familial Partial, Type 2; Hutchinson-gilford Progeria Syndrome; Muscular Dystrophy, Limb-girdle, Type 1b; Cardiomyopathy, Dilated, 1a; Cardiomyopathy, Dilated, With Hypergonadotropic Hypogonadism