Rabbit LITAF Polyclonal Antibody | anti-LITAF antibody

LITAF (LPS-induced TNF-alpha Factor, Lipopolysaccharide-induced Tumor Necrosis Factor-alpha Factor, PIG7, SIMPLE, TP53I7) (APC)

Cat. Num. AAA6424836

• Gene Names: LITAF; PIG7; SIMPLE; TP53I7
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: LITAF; Polyclonal Antibody; LITAF (LPS-induced TNF-alpha Factor; Lipopolysaccharide-induced Tumor Necrosis Factor-alpha Factor; PIG7; SIMPLE; TP53I7) (APC); anti-LITAF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes LITAF. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-LITAF antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human LITAF.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using LITAF antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded mouse lung using LITAF antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human endometrial cancer using LITAF antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human thyroid cancer using LITAF antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using LITAF antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-LITAF antibody

Antibodies; Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 210147505
• NCBI GeneID: 9516
• NCBI Accession #: NP_001129944.1
• NCBI GenBank Nucleotide #: NM_001136472.1
• UniProt Accession #: Q99732; Q05DW0; Q9C0L6; D3DUG1; G5E9K0
• Molecular Weight: 15,824 Da
• NCBI Official Full Name: lipopolysaccharide-induced tumor necrosis factor-alpha factor isoform 1
• NCBI Official Synonym Full Names: lipopolysaccharide-induced TNF factor
• NCBI Official Symbol: LITAF
• NCBI Official Synonym Symbols: PIG7; SIMPLE; TP53I7
• NCBI Protein Information: lipopolysaccharide-induced tumor necrosis factor-alpha factor; LPS-induced TNF-alpha factor; lipopolysaccharide-induced TNF-alpha factor; p53-induced gene 7 protein; small integral membrane protein of lysosome/late endosome; tumor protein p53 inducible pr
• UniProt Protein Name: Lipopolysaccharide-induced tumor necrosis factor-alpha factor
• Protein Family: Lipopolysaccharide-induced tumor necrosis factor-alpha factor
• UniProt Gene Name: LITAF
• UniProt Synonym Gene Names: PIG7; SIMPLE; LPS-induced TNF-alpha factor
• UniProt Entry Name: LITAF_HUMAN

NCBI Description

Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppressor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

LITAF: Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF- alpha) gene expression. Interacts with NEDD4. Interacts with WWOX. Isoform 2 may interact with STAT6. By bacterial lipopolysaccharides (LPS) or p53/TP53. In monocytes by the Bacillus Calmette-Guerin (BCG). Ubiquitously and abundantly expressed. Expressed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 16p13.13

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; lysosomal membrane; cytoplasm; plasma membrane

Molecular Function: protein binding; signal transducer activity; WW domain binding

Biological Process: regulation of transcription from RNA polymerase II promoter; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; apoptosis; negative regulation of NF-kappaB import into nucleus; signal transduction; aging; regulation of cytokine production

Disease: Charcot-marie-tooth Disease, Demyelinating, Type 1c