Rabbit anti-Human LIPE Polyclonal Antibody | anti-LIPE antibody

LIPE, CT (LIPE, Hormone-sensitive lipase) (FITC)

Cat. Num. AAA6316419

• Gene Names: LIPE; HSL; LHS
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: LIPE; Polyclonal Antibody; CT (LIPE; Hormone-sensitive lipase) (FITC); Hormone-sensitive lipase; anti-LIPE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LIPE
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-LIPE antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: LIPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 828~858 amino acids from the C-terminal region of human LIPE.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of LIPE Antibody (C-term) in HL-60 cell line lysates (35ug/lane). LIPE (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with LUC7L Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(LIPE Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-LIPE antibody

LIPE has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

Product Categories/Family for anti-LIPE antibody

Antibodies; Enzymes; Lipase

References

Bezaire, V., et al. FEBS Lett. 583(18):3045-3049(2009). Chen, H.H., et al. Am. J. Clin. Nutr. 90(2):255-262(2009). Kuzmin, A., et al. Biol. Reprod. 81(2):319-326(2009). Bezaire, V., et al. J. Biol. Chem. 284(27):18282-18291(2009). Drenos, F., et al. Hum. Mol. Genet. 18(12):2305-2316(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 21328446
• NCBI GeneID: 3991
• NCBI Accession #: NP_005348.2
• NCBI GenBank Nucleotide #: NM_005357.2
• UniProt Accession #: Q05469; Q3LRT2; Q6NSL7
• Molecular Weight: 116,598 Da
• NCBI Official Full Name: hormone-sensitive lipase
• NCBI Official Synonym Full Names: lipase, hormone-sensitive
• NCBI Official Symbol: LIPE
• NCBI Official Synonym Symbols: HSL; LHS
• NCBI Protein Information: hormone-sensitive lipase; hormone-sensitive lipase testicular isoform
• UniProt Protein Name: Hormone-sensitive lipase
• Protein Family: Hormone-sensitive lipase
• UniProt Gene Name: LIPE
• UniProt Synonym Gene Names: HSL
• UniProt Entry Name: LIPS_HUMAN

NCBI Description

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008]

Uniprot Description

HSL: hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.

Protein type: EC 3.1.1.79; Lipase

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: lipid particle; caveola; cytosol

Molecular Function: triacylglycerol lipase activity; hormone-sensitive lipase activity; protein binding; protein kinase binding

Biological Process: cholesterol metabolic process; triacylglycerol catabolic process; diacylglycerol catabolic process; protein amino acid phosphorylation; lipid catabolic process; long-chain fatty acid catabolic process

Disease: Abdominal Obesity-metabolic Syndrome 4