Rabbit LIN7B Polyclonal Antibody | anti-LIN7B antibody

LIN7B Antibody

Cat. Num. AAA9630656

• Gene Names: LIN7B; MALS2; VELI2; LIN-7B; MALS-2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: LIN7B; Polyclonal Antibody; LIN7B Antibody; hVeli2; hLin7B; Lin 7 homolog B C elegans; LIN 7B; Lin 7b protein likely ortholog of mouse LIN 7B mammalian LIN 7 protein 2; Lin-7B; LIN7B_HUMAN; MALS 2; MALS-2; MALS2; Mammalian lin seven protein 2; Mammalian lin-seven protein 2; Protein lin 7 homolog B; Protein lin-7 homolog B; VELI 2; Veli-2; VELI2; Vertebrate lin 7 homolog 2; Vertebrate lin-7 homolog 2; anti-LIN7B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: LIN7B Antibody detects endogenous levels of LIN7B.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-LIN7B antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human LIN7B.
• Conjugation: Unconjugated
• Similarity: The kinase interacting site is required for proper delivery of ERBB2 to the basolateral membrane. The PDZ domain regulates endocytosis and recycling of the receptor at the membrane. The L27 domain mediates interaction with CASK and is involved in the formation of multimeric complexes and the association of LIN7 to membranes. Belongs to the lin-7 family.
• Subunit Structure: Forms two exclusive ternary complexes with CASK and APBA1 or CASKIN1 (By similarity). Forms a heterotrimeric complex composed of MMP5, LIN7B and PATJ; the N-terminal L27 domain of MPP5 interacts with the L27 domain of PATJ and the C-terminal L27 domain of MPP5 interacts with the L27 domain of LIN7B (By similarity). Forms a heterotrimeric complex with DLG1 and CASK via their L27 domains. Interacts with DLG4 and GRIN2B as well as CDH1 and CTNNB1, the channels KCNJ12/Kir2.2, KCNJ4/Kir2.3 and probably KCNJ2/Kir2.1 and SLC6A12/BGT-1 via its PDZ domain. The association of LIN7A with cadherin and beta-catenin is calcium-dependent, occurs at synaptic junctions and requires the actin cytoskeleton. Interacts with EGFR, ERBB2, ERBB3 and ERBB4 with both PDZ and KID domains. Associates with KIF17 via APBA1 (By similarity). Interacts with ASIC3. Interacts with TOPK. Interacts with RTKN. Interacts with APBA1 (By similarity). Interacts with MPP7. Interacts with DLG2 (By similarity). Interacts with DLG3 (By similarity).
• Function: Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. May increase the amplitude of ASIC3 acid-evoked currents by stabilizing the channel at the cell surface (By similarity).
• Note: Mainly basolateral in renal epithelial cells.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-LIN7B antibody

Cell membrane; Peripheral membrane protein; Basolateral cell membrane; Cell junction; Synapse; Postsynaptic density membrane; Tight junction; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 11545920
• NCBI GeneID: 64130
• NCBI Accession #: NP_071448.1
• NCBI GenBank Nucleotide #: NM_022165.2
• UniProt Accession #: Q9HAP6
• Molecular Weight: 15,620 Da
• NCBI Official Full Name: protein lin-7 homolog B
• NCBI Official Synonym Full Names: lin-7 homolog B (C. elegans)
• NCBI Official Symbol: LIN7B
• NCBI Official Synonym Symbols: MALS2; VELI2; LIN-7B; MALS-2
• NCBI Protein Information: protein lin-7 homolog B; hLin7B; hVeli2; mammalian lin-seven protein 2; veli-2; vertebrate lin-7 homolog 2
• UniProt Protein Name: Protein lin-7 homolog B
• Protein Family: Protein
• UniProt Gene Name: LIN7B
• UniProt Synonym Gene Names: MALS2; VELI2; Lin-7B; hLin7B; MALS-2; Veli-2; hVeli2
• UniProt Entry Name: LIN7B_HUMAN

Uniprot Description

LIN7B: Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. May increase the amplitude of ASIC3 acid-evoked currents by stabilizing the channel at the cell surface. Belongs to the lin-7 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: postsynaptic membrane; neuron projection; tight junction; basolateral plasma membrane; postsynaptic density; synapse; intercellular junction

Molecular Function: protein domain specific binding

Biological Process: asymmetric protein localization; protein transport; exocytosis; neurotransmitter secretion; maintenance of epithelial cell polarity