Rabbit anti-Human, Mouse LIMK2 Polyclonal Antibody | anti-LIMK2 antibody

LIMK2 Polyclonal Antibody

Cat. Num. AAA3009482

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: LIMK2; Polyclonal Antibody; LIMK2 Polyclonal Antibody; LIM domain kinase 2; LIMK 2; anti-LIMK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LIMK2 polyclonal antibody detects endogenous levels of LIMK2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 686

• Applicable Applications for anti-LIMK2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human LIMK2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of LIMK2 polyclonal antibody)

Related Product Information for anti-LIMK2 antibody

Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, incuding LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and Cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morpho-genesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and it is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.

NCBI and Uniprot Product Information

• NCBI GI #: 73390140
• NCBI GeneID: 3985
• NCBI Accession #: NP_001026971.1
• NCBI GenBank Nucleotide #: NP_001026971.1
• UniProt Accession #: P53671
• Molecular Weight: ~ 80kDa
• NCBI Official Full Name: LIM domain kinase 2 isoform 1
• NCBI Official Synonym Full Names: LIM domain kinase 2
• NCBI Official Symbol: LIMK2
• NCBI Protein Information: LIM domain kinase 2
• UniProt Protein Name: LIM domain kinase 2
• Protein Family: LIM domain kinase
• UniProt Gene Name: LIMK2
• UniProt Synonym Gene Names: LIMK-2
• UniProt Entry Name: LIMK2_HUMAN

NCBI Description

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LIMK2: a TKL kinase of the LISK family that regulates actin dynamics. Contains two N-terminal LIM motifs. Phosphorylated and activated by PAK1 and ROCK, downstream effectors of Rho. Phosphorylates cofilin, inhibiting its actin-depolymerizing activity. May contributes to Rho-induced reorganization of the actin cytoskeleton. Two alternative splice variants have been identified.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; LISK family; LIMK subfamily

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: cis-Golgi network; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; zinc ion binding; protein heterodimerization activity; ATP binding

Biological Process: spermatogenesis; phosphorylation; protein amino acid phosphorylation