Rabbit anti-Human, Mouse LILRB1 Polyclonal Antibody | anti-LILRB1 antibody

Anti-LILRB1 Antibody

Cat. Num. AAA1753578

• Gene Names: LILRB1; ILT2; LIR1; MIR7; CD85J; ILT-2; LIR-1; MIR-7
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: LILRB1; Polyclonal Antibody; Anti-LILRB1 Antibody; ILT2; LIR1; MIR7; Leukocyte immunoglobulin-like receptor subfamily B member 1; LIR-1; Leukocyte immunoglobulin-like receptor 1; CD85 antigen-like family member J; Immunoglobulin-like transcript 2; ILT-2; Monocyte/macrophage immunoglobulin-like receptor 7; MIR-7; CD antigen CD85j; leukocyte immunoglobulin like receptor B1; anti-LILRB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for LILRB1 detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• Applicable Applications for anti-LILRB1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml|Human, Mouse|; IHC-P: 2-5ug/ml|Human|; FC/FACS/FCM: 1-3ug/1x106 cells|Human|; Direct ELISA: 0.1-0.5ug/ml|Human|
• Immunogen: E Coli-derived human LILRB1 recombinant protein (Position: Q347-Q442).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-LILRB1 antibody

Leukocyte immunoglobulin-like receptor subfamily B member 1 is a protein that in humans is encoded by the LILRB1 gene. This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13. 4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.

References

1. Andre, P., Biassoni, R., Colonna, M., Cosman, D., Lanier, L. L., Long, E. O., Lopez-Botet, M., Moretta, A., Moretta, L., Parham, P., Trowsdale, J., Vivier, E., Wagtmann, N., Wilson, M. J. New nomenclature for MHC receptors. Nature Immun. 2: 661 only, 2001.
2. Banham, A. H., Colonna, M., Cella, M., Micklem, K. J., Pulford, K., Willis, A. C., Mason, D. Y. Identification of the CD85 antigen as ILT2, an inhibitory MHC class I receptor of the immunoglobulin superfamily. J. Leukoc. Biol. 65: 841-845, 1999.
3. Borges, L., Hsu, M. -L., Fanger, N., Kubin, M., Cosman, D. A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules. J. Immun. 159: 5192-5196, 1997.

NCBI and Uniprot Product Information

• NCBI GI #: 612149795
• NCBI GeneID: 10859
• NCBI Accession #: NP_001075108.2
• NCBI GenBank Nucleotide #: NM_001081639.3
• UniProt Accession #: Q8NHL6; O75024; O75025; Q8NHJ9; Q8NHK0; A2IXV4; A8MXT0
• Molecular Weight: 70,819 Da
• NCBI Official Full Name: leukocyte immunoglobulin-like receptor subfamily B member 1 isoform 4
• NCBI Official Synonym Full Names: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
• NCBI Official Symbol: LILRB1
• NCBI Official Synonym Symbols: ILT2; LIR1; MIR7; CD85J; ILT-2; LIR-1; MIR-7
• NCBI Protein Information: leukocyte immunoglobulin-like receptor subfamily B member 1; Ig-like transcript 2; CD85 antigen-like family member J; monocyte/macrophage immunoglobulin-like receptor 7; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform
• UniProt Protein Name: Leukocyte immunoglobulin-like receptor subfamily B member 1
• Protein Family: Leukocyte immunoglobulin-like receptor subfamily
• UniProt Gene Name: LILRB1
• UniProt Synonym Gene Names: ILT2; LIR1; MIR7; LIR-1; Leukocyte immunoglobulin-like receptor 1; ILT-2; MIR-7
• UniProt Entry Name: LIRB1_HUMAN

NCBI Description

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

LILRB1: Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis. Interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: cytoplasm; extracellular region; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein homodimerization activity; MHC class I receptor activity; MHC class I protein binding; HLA-A specific inhibitory MHC class I receptor activity; SH2 domain binding; HLA-B specific inhibitory MHC class I receptor activity; protein phosphatase 1 binding

Biological Process: immune response-inhibiting cell surface receptor signaling pathway; regulation of immune response; negative regulation of calcium ion transport; negative regulation of mononuclear cell proliferation; positive regulation of apoptosis; negative regulation of endocytosis; response to virus; negative regulation of natural killer cell mediated cytotoxicity; signal transduction; negative regulation of cytokine secretion during immune response; negative regulation of interferon-gamma biosynthetic process; negative regulation of cell cycle; negative regulation of serotonin secretion; negative regulation of alpha-beta T cell activation; negative regulation of T cell proliferation; T cell proliferation during immune response; receptor internalization; negative regulation of interferon-gamma production; negative regulation of T cell mediated cytotoxicity; interferon-gamma production; negative regulation of tumor necrosis factor biosynthetic process; Fc receptor mediated inhibitory signaling pathway; positive regulation of cytolysis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of defense response to virus by host; defense response to virus