Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using LIF antibody at dilution of 1:100 (40x lens).)
Rabbit anti-Human LIF Polyclonal Antibody | anti-LIF antibody
LIF Polyclonal Antibody
Gene Names
LIF; CDF; DIA; HILDA; MLPLI
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
LIF; Polyclonal Antibody; LIF Polyclonal Antibody; HILDA; Leukemia inhibitory factor; Differentiationstimulating factor; Melanomaderived LPL inhibitor; INN=Emfilermin; Flags: Precursor; anti-LIF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
88
Applicable Applications for anti-LIF antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot (WB): 1:200-1:500
Immunohistochemistry (IHC): 1:50-1:200
Flow Cytometry (FC/FACS): 1:20-1:50
Immunohistochemistry (IHC): 1:50-1:200
Flow Cytometry (FC/FACS): 1:20-1:50
Immunogen
A synthetic peptide of human LIF
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using LIF antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using LIF antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-LIF antibody
Leukemia Inhibitory Factor (LIF) is a 20 kDa pleiotrophic factor belonging to the IL6 superfamily of cytokines (1). LIF is expressed in a number of tissues and cell types. The LIF receptor is a heterodimer comprised of LIFR (gp190) and gp130, a common signal transducer for IL6type cytokines (1). Depending on cell type and context, LIF/LIFR can activate Erk, PI3K, and Jak1/Stat1/3 pathways (1,2). LIF has a diverse array of biological activities. Murine embryonic stem cells are dependent on LIF for pluripotency and selfrenewal in vitro (1). Exerciseinduced LIF secretion in muscle induces myoblast proliferation, suggesting that LIF may play a role in exerciseinduced muscle hypertrophy (2). LIF also negatively regulates Th2 and Th17 cell differentiation (3,4)
References
1. Mathieu, M.E. et al. (2012) Stem Cell Rev 8, 115. 2. Broholm, C. and Pedersen, B.K. (2010) Exerc Immunol Rev 16, 7785 3. Cao, W. et al. (2011) Immunity 35, 27384. 4. Ullah, U. et al. (2012) Sci Rep 2, 464.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22kDa
NCBI Official Full Name
leukemia inhibitory factor isoform 2
NCBI Official Synonym Full Names
leukemia inhibitory factor
NCBI Official Symbol
LIF
NCBI Official Synonym Symbols
CDF; DIA; HILDA; MLPLI
NCBI Protein Information
leukemia inhibitory factor; D factor; human interleukin in DA cells; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; differentiation-stimulating factor; differentiation inhibitory activity
UniProt Protein Name
Leukemia inhibitory factor
Protein Family
UniProt Gene Name
LIF
UniProt Synonym Gene Names
HILDA; LIF; D factor; MLPLI
UniProt Entry Name
LIF_HUMAN
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Product Notes
The LIF lif (Catalog #AAA127798) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LIF Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LIF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot (WB): 1:200-1:500 Immunohistochemistry (IHC): 1:50-1:200 Flow Cytometry (FC/FACS): 1:20-1:50. Researchers should empirically determine the suitability of the LIF lif for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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