Immunohistochemistry (IHC)
(MBS9607791 at 1/100 staining Mouse testis tissue by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigenretrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°Covernight. An HRP conjugated anti-Rabbit antibody was usedas the secondary antibody.)
Rabbit LHB Polyclonal Antibody | anti-LHB antibody
LHB Antibody
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
IF: 1:100-1:500
ICC: 1:100-1:500
IHC: 1:50-1:200
ELISA (peptide): 1:20000-1:40000
The optimal dilutions should be determined by the end user.
Important note: For western blots, incubate membrane with diluted Ab in 5% w/v milk, 1X TBS , 0.1% TweenR20 AT 4°C with gentle shaking overnight.
Immunohistochemistry (IHC)
(MBS9607791 at 1/100 staining Mouse testis tissue by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigenretrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°Covernight. An HRP conjugated anti-Rabbit antibody was usedas the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9607791 at 1/100 staining Mouse testis tissue by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigenretrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°Covernight. An HRP conjugated anti-Rabbit antibody was usedas the secondary antibody.)
Immunofluorescence (IF)
(MBS9607791 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9607791 1:200) and mouse anti-beta tubulin Ab( MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9607791 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9607791 1:200) and mouse anti-beta tubulin Ab( MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts of Mouse pituitary, using LHB antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts of Mouse pituitary, using LHB antibody. The lane on the left is treated with the antigen-specific peptide.)
Function: Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Subunit Structure: Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.
Similarity: Belongs to the glycoprotein hormones subunit beta family.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the glycoprotein hormone beta chain family and encodes the beta subunit of luteinizing hormone (LH). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism. [provided by RefSeq, Jul 2008]
Uniprot Description
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
Research Articles on LHB
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Product Notes
The LHB lhb (Catalog #AAA9607791) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LHB Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LHB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000IF: 1:100-1:500
ICC: 1:100-1:500
IHC: 1:50-1:200
ELISA (peptide): 1:20000-1:40000 The optimal dilutions should be determined by the end user.
Important note: For western blots, incubate membrane with diluted Ab in 5% w/v milk, 1X TBS, 0.1% TweenR20 AT 4°C with gentle shaking overnight. Researchers should empirically determine the suitability of the LHB lhb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LHB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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