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Immunohistochemistry (IHC) (IHC image of MBS7134663 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Rabbit anti-Human LGALS4 Polyclonal Antibody | anti-LGALS4 antibody

LGALS4 Antibody

Gene Names
LGALS4; GAL4; L36LBP
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunofluorescence
Purity
Antigen Affinity Purified
Synonyms
LGALS4; Polyclonal Antibody; LGALS4 Antibody; Galectin-4 (Gal-4) (Antigen NY-CO-27) (L-36 lactose-binding protein) (L36LBP) (Lactose-binding lectin 4); anti-LGALS4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Applicable Applications for anti-LGALS4 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
IHC: 1:20-1:200
IF: 1:50-1:200
Immunogen
Recombinant Human Galectin-4 protein (1-323AA)
Immunogen Species
Human
Conjugate
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(IHC image of MBS7134663 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7134663 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunofluorescence (IF)

(Immunofluorescence staining of A549 cells with MBS7134663 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

Immunofluorescence (IF) (Immunofluorescence staining of A549 cells with MBS7134663 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Product Categories/Family for anti-LGALS4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,941 Da
NCBI Official Full Name
galectin-4
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 4
NCBI Official Symbol
LGALS4
NCBI Official Synonym Symbols
GAL4; L36LBP
NCBI Protein Information
galectin-4; gal-4; galectin 4; antigen NY-CO-27; lactose-binding lectin 4; L-36 lactose-binding protein
UniProt Protein Name
Galectin-4
Protein Family
UniProt Gene Name
LGALS4
UniProt Synonym Gene Names
Gal-4; L36LBP
UniProt Entry Name
LEG4_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

galectin-4: Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: plasma membrane; cytosol

Molecular Function: carbohydrate binding

Biological Process: cell adhesion

Research Articles on LGALS4

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Product Notes

The LGALS4 lgals4 (Catalog #AAA7134663) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LGALS4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LGALS4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). IHC: 1:20-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the LGALS4 lgals4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LGALS4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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