Rabbit anti-Human Leptin Receptor Polyclonal Antibody | anti-LEPR antibody

Leptin Receptor, NT (LEPR, LEP-R, OB Receptor, OB-R, HuB219, CD295, DB, OBR) (FITC)

Cat. Num. AAA6484946

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Leptin Receptor; Polyclonal Antibody; NT (LEPR; LEP-R; OB Receptor; OB-R; HuB219; CD295; DB; OBR) (FITC); anti-LEPR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LEPR.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-LEPR antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human LEPR (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Leptin Receptor (LEPR) Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LEPR antibody detected the LEPR protein (arrow).)

Flow Cytometry (FC/FACS)

(Leptin Receptor (LEPR) Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-LEPR antibody

Antibodies; Obesity Proteins

References

1. Schroth, M., et al., J. Clin. Endocrinol. Metab. 88(11):5497-5501 (2003). 2. Couturier, C., et al., J. Biol. Chem. 278(29):26604-26611 (2003). 3. Gavrila, A., et al., J. Clin. Endocrinol. Metab. 88(6):2838-2843 (2003). 4. Yannakoulia, M., et al., J. Clin. Endocrinol. Metab. 88(4):1730-1736 (2003). 5. Kado, N., et al., Hum. Reprod. 18(4):715-720 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 82569700
• UniProt Accession #: P48357
• Molecular Weight: 96kDa
• NCBI Official Full Name: LEPR, partial
• UniProt Protein Name: Leptin receptor
• Protein Family: Leptin receptor
• UniProt Gene Name: LEPR
• UniProt Synonym Gene Names: DB; OBR; LEP-R; OB-R
• UniProt Entry Name: LEPR_HUMAN

Uniprot Description

LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: integral to membrane; plasma membrane; extracellular region; receptor complex

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; transmembrane receptor activity

Biological Process: cholesterol metabolic process; cell surface receptor linked signal transduction; negative regulation of hydrolase activity; multicellular organismal development; negative regulation of gluconeogenesis; cytokine and chemokine mediated signaling pathway; energy reserve metabolic process

Disease: Leptin Receptor Deficiency