Rabbit Leptin Polyclonal Antibody | anti-LEP antibody

Leptin antibody (biotin)

Cat. Num. AAA534428

• Gene Names: LEP; OB; OBS; LEPD
• Reactivity: Murine leptin
• Applications: ELISA, Western Blot
• Synonyms: Leptin; Polyclonal Antibody; Leptin antibody (biotin); Polyclonal Leptin; Anti-Leptin; Obesity; OB; anti-LEP antibody

• Host: Rabbit
• Reactivity: Murine leptin
• Clonality: Polyclonal
• Specificity: Murine leptin
• Form/Format: Lyophilized from PBS, pH 7.2. Reconstitute in sterile PBS with 0.1% BSA to a concentration of at least 50ug/ml.
• Sequence Length: 167

• Applicable Applications for anti-LEP antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 0.25-1 ug/ml; WB: 0.1-0.2 ug/ml
• Biological Significance: Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models.
• Tag/Conjugate: Biotin
• Immunogen: Leptin antibody was raised in rabbit using highly pure recombinant murine leptin as the immunogen.
• Preparation and Storage: Store at -20 degree C until reconstitution. Following reconstitution product may be stored at 4 degree C in the short term. For long term storage aliquot and freeze at -20 degree C. Avoid repeated freeze/thaw cycles.

Related Product Information for anti-LEP antibody

Rabbit polyclonal Leptin antibody (biotin)

Product Categories/Family for anti-LEP antibody

Hormones & Steroids; Conjugated Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4557715
• NCBI GeneID: 3952
• NCBI Accession #: NP_000221.1
• NCBI GenBank Nucleotide #: NM_000230.2
• UniProt Accession #: P41159; O15158; Q56A88
• Molecular Weight: 16.2 kDa (predicted detection band MW)
• NCBI Official Full Name: leptin
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: LEP
• NCBI Official Synonym Symbols: OB; OBS; LEPD
• NCBI Protein Information: leptin
• UniProt Protein Name: Leptin
• Protein Family: Leptin
• UniProt Gene Name: LEP
• UniProt Synonym Gene Names: OB; OBS
• UniProt Entry Name: LEP_HUMAN

NCBI Description

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

Uniprot Description

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.

Protein type: Secreted; Cell development/differentiation; Hormone; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: peptide hormone receptor binding; growth factor activity; hormone activity

Biological Process: circadian rhythm; response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; regulation of steroid biosynthetic process; female pregnancy; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; positive regulation of luteinizing hormone secretion; negative regulation of appetite; response to insulin stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein; response to vitamin E; positive regulation of MAPKKK cascade; regulation of cholesterol absorption; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; central nervous system neuron development; placenta development; positive regulation of cytokine production; cholesterol metabolic process; positive regulation of developmental growth; eating behavior; bile acid metabolic process; glucose metabolic process; adult feeding behavior; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; insulin secretion; glycerol biosynthetic process; energy reserve metabolic process; response to hypoxia; hormone metabolic process; regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway; positive regulation of follicle-stimulating hormone secretion; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis

Disease: Leptin Deficiency