Rabbit anti-Human LEO1 Polyclonal Antibody | anti-LEO1 antibody

LEO1, Phosphorylated (Ser10) (RDL, RNA Polymerase-associated Protein LEO1, Replicative Senescence Down-regulated leo1-like Protein, RDL) (FITC)

Cat. Num. AAA6484880

• Gene Names: LEO1; RDL
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LEO1; Polyclonal Antibody; Phosphorylated (Ser10) (RDL; RNA Polymerase-associated Protein LEO1; Replicative Senescence Down-regulated leo1-like Protein; RDL) (FITC); anti-LEO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LEO1 when phosphorylated at Ser10.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-LEO1 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser10 of human LEO1 (KLH).
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-LEO1-pS10 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-LEO1 antibody

Antibodies; Enzymes; Polymerase

References

1. Olsen JV, et al. (2006). Cell 127, 635-48.

NCBI and Uniprot Product Information

• NCBI GI #: 20270337
• NCBI GeneID: 123169
• NCBI Accession #: NP_620147
• NCBI GenBank Nucleotide #: NM_138792
• UniProt Accession #: Q8WVC0; Q96N99
• Molecular Weight: 68,004 Da
• NCBI Official Full Name: RNA polymerase-associated protein LEO1 isoform 1
• NCBI Official Synonym Full Names: Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• NCBI Official Symbol: LEO1
• NCBI Official Synonym Symbols: RDL
• NCBI Protein Information: RNA polymerase-associated protein LEO1; replicative senescence down-regulated leo1-like protein
• UniProt Protein Name: RNA polymerase-associated protein LEO1
• Protein Family: RNA polymerase-associated protein
• UniProt Gene Name: LEO1
• UniProt Synonym Gene Names: RDL
• UniProt Entry Name: LEO1_HUMAN

Uniprot Description

LEO1: a component of the PAF1 multifunctional complex involved in initiation and elongation, histone methylation and RNA processing. The PAF1 complex interacts with POLR2A. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts. The PAF1 complex consists of at least of CDC73, PAF1, LEO1 and CTR9. Interacts with CDC73. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: nucleoplasm; nucleolus

Molecular Function: protein binding

Biological Process: histone monoubiquitination; Wnt receptor signaling pathway; negative regulation of myeloid cell differentiation; endodermal cell fate commitment; transcription, DNA-dependent; positive regulation of RNA elongation from RNA polymerase II promoter; stem cell maintenance; histone H2B ubiquitination; positive regulation of mRNA 3'-end processing; mRNA polyadenylation; positive regulation of transcription from RNA polymerase II promoter