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Western Blot (WB) (Anti-Lefty2 Antibodyat 1:1000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human, mouse Lefty2 Polyclonal Antibody | anti-Lefty2 antibody

Lefty2 Antibody

Gene Names
Lefty2; Ebaf; Lefta; Leftb; AV214969; 6030463A22Rik
Reactivity
Human, mouse
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Lefty2; Polyclonal Antibody; Lefty2 Antibody; Left-right determination factor 2; Left-right determination factor B; Protein lefty-2; Protein lefty-B; Leftb; anti-Lefty2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Lefty2 antibody is generated from a rabbit immunized with a recombinant protein.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
368
Applicable Applications for anti-Lefty2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:2000
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-Lefty2 Antibodyat 1:1000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-Lefty2 Antibodyat 1:1000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-Lefty2 Antibodyat 1:2000 dilution + mouse heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-Lefty2 Antibodyat 1:2000 dilution + mouse heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Lefty2 antibody
Required for left-right asymmetry determination of organ systems in mammals.
Product Categories/Family for anti-Lefty2 antibody
References
Meno C.,et al.Genes Cells 2:513-524(1997).
Carninci P.,et al.Science 309:1559-1563(2005).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41175
NCBI Official Full Name
left-right determination factor 2
NCBI Official Synonym Full Names
left-right determination factor 2
NCBI Official Symbol
Lefty2
NCBI Official Synonym Symbols
Ebaf; Lefta; Leftb; AV214969; 6030463A22Rik
NCBI Protein Information
left-right determination factor 2
UniProt Protein Name
Left-right determination factor 2
UniProt Gene Name
Lefty2
UniProt Synonym Gene Names
Leftb
UniProt Entry Name
LFTY2_MOUSE

Uniprot Description

LEFTY2: Required for left-right (L-R) asymmetry determination of organ systems in mammals. May play a role in endometrial bleeding. Defects in LEFTY2 are the cause of left-right axis malformations (LRAM). The defect includes left pulmonary isomerism, with cardiac anomalies characterized by complete atrioventricular canal defect and hypoplastic left ventricle, and interrupted inferior vena cava. Belongs to the TGF-beta family.

Protein type: Cytokine; Secreted, signal peptide; Ligand, receptor tyrosine kinase; Secreted

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; multicellular organismal development; regulation of MAPKKK cascade; cell growth; cell development; growth

Research Articles on Lefty2

Similar Products

Product Notes

The Lefty2 lefty2 (Catalog #AAA9209553) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Lefty2 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Lefty2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2000. Researchers should empirically determine the suitability of the Lefty2 lefty2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lefty2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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