Rabbit LCP1 Polyclonal Antibody | anti-LCP1 antibody

LCP1 (Lymphocyte Cytosolic Protein 1, LPL, CP64, PLS2, LC64P, L-PLASTIN) (AP)

Cat. Num. AAA6424538

• Gene Names: LCP1; LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: LCP1; Polyclonal Antibody; LCP1 (Lymphocyte Cytosolic Protein 1; LPL; CP64; PLS2; LC64P; L-PLASTIN) (AP); anti-LCP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes LCP1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-LCP1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human LCP1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using LCP1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using LCP1 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-LCP1 antibody

Antibodies; T Cells

NCBI and Uniprot Product Information

• NCBI GI #: 119629163
• NCBI GeneID: 3936
• UniProt Accession #: P13796; Q5TBN4; B2R613
• Molecular Weight: 70,288 Da
• NCBI Official Full Name: lymphocyte cytosolic protein 1 (L-plastin), isoform CRA_a
• NCBI Official Synonym Full Names: lymphocyte cytosolic protein 1 (L-plastin)
• NCBI Official Symbol: LCP1
• NCBI Official Synonym Symbols: LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
• NCBI Protein Information: plastin-2; LCP-1; plastin 2; epididymis secretory protein Li 37; Lymphocyte cytosolic protein-1 (plasmin); bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin)); L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P)
• UniProt Protein Name: Plastin-2
• Protein Family: Larval cuticle protein
• UniProt Gene Name: LCP1
• UniProt Synonym Gene Names: PLS2; LCP-1
• UniProt Entry Name: PLSL_HUMAN

NCBI Description

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq, Jul 2008]

Uniprot Description

L-plastin: Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69. Monomer. Interacts with AIF1. Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T- lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: ruffle; extracellular space; focal adhesion; cytoplasm; stress fiber; actin filament bundle; plasma membrane; actin filament; cytosol; filopodium; actin cytoskeleton; phagocytic cup

Molecular Function: actin filament binding; identical protein binding; calcium ion binding; actin binding

Biological Process: actin filament bundle formation; cell migration; organ regeneration; regulation of intracellular protein transport; T cell activation during immune response