Western Blot (WB)
(Western Blot analysis of LAYN in Jurkat and K562 cell lysates in order using LAYN Rabbit pAb at dilution 1/700)
Rabbit anti-Human, Mouse LAYN Polyclonal Antibody | anti-LAYN antibody
LAYN Rabbit pAb
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
LAYN; Polyclonal Antibody; LAYN Rabbit pAb; layilin; anti-LAYN antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
0.8mg/ml (varies by lot)
Applicable Applications for anti-LAYN antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:100
IHC-P: 1:50-1:100
Immunogen
Synthetic peptide of human LAYN
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western Blot analysis of LAYN in Jurkat and K562 cell lysates in order using LAYN Rabbit pAb at dilution 1/700)
Western Blot (WB)
(Western Blot analysis of LAYN in Jurkat and K562 cell lysates in order using LAYN Rabbit pAb at dilution 1/700)
Immunohistochemistry (IHC)
(Immunohistochemistry of LAYN in paraffin-embedded Human esophagus cancer tissue using LAYN Rabbit pAb at dilution 1/50. Predicted cell location: Cell membrane)
Immunohistochemistry (IHC)
(Immunohistochemistry of LAYN in paraffin-embedded Human esophagus cancer tissue using LAYN Rabbit pAb at dilution 1/50. Predicted cell location: Cell membrane)
Related Product Information for anti-LAYN antibody
Layilin is a recently cloned ?55-kDa membrane-binding partner for talin (Borowsky and Hynes, 1998) that is widely expressed in different cell types and tissue extracts. It is found in peripheral ruffles of spreading cells and is recruited to membrane ruffles in cells induced to migrate in in vitro wounding experiments. Layilin colocalizes with talin in ruffles and binds to talin's ?50-kDa head domain (amino acids 280-435). Other parts of talin (the ?220-kDa tail fragment) can bind beta-integrin cytoplasmic tails, vinculin, and F-actin and hence form an integrin-cytoskeleton linkage at sites of cell-substratum contact. Layilin's extracellular domain is homologous with the carbohydrate-recognition domains (CRD) of C-type lectins. Receptor for hyaluronate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
382
NCBI Official Full Name
Layilin
NCBI Official Synonym Full Names
layilin
NCBI Official Symbol
LAYN
NCBI Protein Information
layilin
UniProt Protein Name
Layilin
Protein Family
UniProt Gene Name
LAYN
UniProt Entry Name
LAYN_HUMAN
Uniprot Description
LAYN: Receptor for hyaluronate. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q23.1
Cellular Component: ruffle; cell surface; focal adhesion; integral to membrane
Molecular Function: hyaluronic acid binding; carbohydrate binding
Research Articles on LAYN
Similar Products
Product Notes
The LAYN layn (Catalog #AAA4760616) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LAYN Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LAYN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:50-1:100. Researchers should empirically determine the suitability of the LAYN layn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAYN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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