Rabbit LAT Polyclonal Antibody | anti-LAT antibody

LAT (Phospho-Tyr220) Antibody

Cat. Num. AAA9508522

• Gene Names: LAT; LAT1; pp36; IMD52
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: LAT; Polyclonal Antibody; LAT (Phospho-Tyr220) Antibody; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; p36-38; anti-LAT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: LAT (Phospho-Tyr220) antibody detects endogenous levels of LAT only when phosphorylated at Tyr220.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 261

• Applicable Applications for anti-LAT antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:Y220; M:Y195; R:Y195
• Immunogen: The antiserum was produced against synthesized phospho-peptide derived from human LAT around the phosphorylation site of Tyr220.
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using LAT (Phospho-Tyr220) Antibody)

Western Blot (WB)

(Western blot analysis of lysates from Jurkat cells treated with UV, using p-LAT (Phospho-Tyr220) antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 62739155
• NCBI GeneID: 27040
• NCBI Accession #: NP_001014987.1
• NCBI GenBank Nucleotide #: NM_001014987.1
• UniProt Accession #: O43561; O43919; B7WPI0; C7C5T6; G5E9K3
• Molecular Weight: 27 kDa
• NCBI Official Full Name: linker for activation of T-cells family member 1 isoform b
• NCBI Official Synonym Full Names: linker for activation of T cells
• NCBI Official Symbol: LAT
• NCBI Official Synonym Symbols: LAT1; pp36; IMD52
• NCBI Protein Information: linker for activation of T-cells family member 1
• UniProt Protein Name: Linker for activation of T-cells family member 1
• Protein Family: Latexin
• UniProt Gene Name: LAT
• UniProt Synonym Gene Names: pp36

NCBI Description

The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.