Rabbit LASS5 Polyclonal Antibody | anti-LASS5 antibody

LASS5 Antibody

Cat. Num. AAA9404101

• Gene Names: CERS5; Trh4; LASS5
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: LASS5; Polyclonal Antibody; LASS5 Antibody; Longevity Assurance Homolog 5; LAG1; TRH4; anti-LASS5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Multiple isoforms of LASS5 are known to exist. This antibody is predicted not to cross-react with the highly homologous LASS6.
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 392

• Applicable Applications for anti-LASS5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 14 amino acid peptide near the amino terminus of the human LASS5.
• Target Name: LASS5
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of LASS5 in SK-N-SH lysate with LASS5 antibody at (A) 1 and (B) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of LASS5 in mouse brain tissue with LASS5 antibody at 2.5 ug/mL.)

Related Product Information for anti-LASS5 antibody

The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. LASS5, also called Trh4, is a 392 amino acid endoplasmic reticulum, multi-pass membrane protein. Functioning as a dihydro-ceramide synthase, LASS5 is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl-ceramide) in a fumonisin B1-independent manner. It uses palmitoyl-CoA as an acyl donor and is involved in the synthesis of C14, C16 and C18-ceramide. LASS5 is the most abundantly expressed and predominant ceramide synthase isoform in lung epithelia. Recent studies show that LASS5 partially correct growth and apoptosis.

Product Categories/Family for anti-LASS5 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 22218345
• NCBI GeneID: 91012
• NCBI Accession #: NP_671723
• NCBI GenBank Nucleotide #: NM_147190.3
• Molecular Weight: 39,259 Da
• NCBI Official Full Name: ceramide synthase 5 isoform 1
• NCBI Official Synonym Full Names: ceramide synthase 5
• NCBI Official Symbol: CERS5
• NCBI Official Synonym Symbols: Trh4; LASS5
• NCBI Protein Information: ceramide synthase 5
• UniProt Protein Name: Ceramide synthase 5
• UniProt Gene Name: CERS5
• UniProt Synonym Gene Names: LASS5; CerS5
• UniProt Entry Name: CERS5_HUMAN

NCBI Description

This gene encodes a protein that belongs to the TLC (TRAM, LAG1 and CLN8 homology domains) family of proteins. The encoded protein functions in the synthesis of ceramide, a lipid molecule that is involved in a several cellular signaling pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

LASS5: May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl- (C16) ceramide) in a fumonisin B1-independent manner.

Protein type: Membrane protein, multi-pass; EC 2.3.1.24; Membrane protein, integral; DNA-binding; Transferase

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; endoplasmic reticulum; integral to membrane

Molecular Function: DNA binding; sphingosine N-acyltransferase activity

Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; ceramide biosynthetic process