Rabbit anti-Human, Mouse LARP1 Polyclonal Antibody | anti-LARP1 antibody
LARP1 Antibody
Gene Names
LARP1; LARP
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
LARP1; Polyclonal Antibody; LARP1 Antibody; 1810024J12Rik; 3110040D16Rik; KIAA0731; La related protein; La ribonucleoprotein domain family; member 1; LARP; MGC19556; mKIAA0731; rCG_32791; RGD1306683; RP23-293I3.7; anti-LARP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
LARP1 Antibody detects endogenous levels of total LARP1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-LARP1 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from Human MYOCD.
Conjugation
Unconjugated
Similarity
The C-terminal region mediates interaction with the mRNA and polysomes (PubMed:24532714, PubMed:26206669, PubMed:28379136). It is required for translational repression of mRNAs with a 5'TOP motif (PubMed:29244122). The N-terminal region mediates interaction with PABPC1. Belongs to the LARP family.
Post Translational Modifications
Phosphorylated on multiple Ser and Thr residues in response to active mTORC1. Phosphorylation is important for interaction with RPTOR and the mTORC1 complex. Phosphorylation promotes dissociation from the 5'UTR of mRNA molecules with a 5'TOP motif.
Subunit Structure
Interacts with PABPC1/PABP. Interacts with EIF4A1. Interacts with RPTOR. Recruited to the active mTORC1 complex via interaction with RPTOR. Inhibition of mTORC1 activity strongly reduces interaction with RPTOR and the mTORC1 complex. Identified in a complex with mRNA, PABPC1, EIF4E and EIF4G1. Found in a complex with PABPC1 and SHFL.
Function
RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability. Interacts on the one hand with the 3' poly-A tails that are present in all mRNA molecules, and on the other hand with the 7-methylguanosine cap structure of mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding ribosomal proteins and several components of the translation machinery. The interaction with the 5' end of mRNAs containing a 5'TOP motif leads to translational repression by preventing the binding of EIF4G1. When mTORC1 is activated, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR) of mRNA. Does not prevent binding of EIF4G1 to mRNAs that lack a 5'TOP motif. Interacts with the free 40S ribosome subunit and with ribosomes, both monosomes and polysomes. Under normal nutrient availability, interacts primarily with the 3' untranslated region (UTR) of mRNAs encoding ribosomal proteins and increases protein synthesis. Associates with actively translating ribosomes and stimulates translation of mRNAs containing a 5'TOP motif, thereby regulating protein synthesis, and as a consequence, cell growth and proliferation. Stabilizes mRNAs species with a 5'TOP motif, which is required to prevent apoptosis.(Microbial infection) Positively regulates the replication of dengue virus (DENV).
Note
Colocalizes with RPTOR and PABPC1 in cytoplasmic granules that resemble stress granules.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Product Categories/Family for anti-LARP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
1096
NCBI Official Full Name
la-related protein 1
NCBI Official Synonym Full Names
La ribonucleoprotein domain family, member 1
NCBI Official Symbol
LARP1
NCBI Official Synonym Symbols
LARP
NCBI Protein Information
la-related protein 1
UniProt Protein Name
La-related protein 1
UniProt Gene Name
LARP1
UniProt Synonym Gene Names
KIAA0731; LARP
UniProt Entry Name
LARP1_HUMAN
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Product Notes
The LARP1 larp1 (Catalog #AAA9629402) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LARP1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LARP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the LARP1 larp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LARP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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