Western Blot (WB)
(Western blot analysis of anti-LAP3(N-term) Pab in HepG2 cell line lysates (35ug/lane). LAP3(arrow) was detected using the purified Pab.)
Rabbit anti-Human LAP3 Polyclonal Antibody | anti-LAP3 antibody
LAP3 Antibody (N-term)
Gene Names
LAP3; LAP; PEPS; LAPEP; HEL-S-106
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
LAP3; Polyclonal Antibody; LAP3 Antibody (N-term); Cytosol aminopeptidase; Leucine aminopeptidase 3; LAP-3; Leucyl aminopeptidase; Peptidase S; Proline aminopeptidase; Prolyl aminopeptidase; LAPEP; PEPS; anti-LAP3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This LAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the N-terminal region of human LAP3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 1.74 (varies by lot)
Sequence Positions
89-119
Sequence Length
519
Applicable Applications for anti-LAP3 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of anti-LAP3(N-term) Pab in HepG2 cell line lysates (35ug/lane). LAP3(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-LAP3(N-term) Pab in HepG2 cell line lysates (35ug/lane). LAP3(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with LAP3 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with LAP3 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-LAP3 antibody
LAP3 is presumably involved in the processing and regular turnover of intracellular proteins. It catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro.
Product Categories/Family for anti-LAP3 antibody
References
Goto,Y.,FEBS Lett. 580 (7), 1833-1838 (2006)
Matsushima,M.,Biochem. Biophys. Res. Commun. 178 (3), 1459-1464 (1991)
Matsushima,M.,Biochem. Biophys. Res. Commun. 178 (3), 1459-1464 (1991)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56166
NCBI Official Full Name
cytosol aminopeptidase
NCBI Official Synonym Full Names
leucine aminopeptidase 3
NCBI Official Symbol
LAP3
NCBI Official Synonym Symbols
LAP; PEPS; LAPEP; HEL-S-106
NCBI Protein Information
cytosol aminopeptidase
UniProt Protein Name
Cytosol aminopeptidase
Protein Family
UniProt Gene Name
LAP3
UniProt Synonym Gene Names
LAPEP; PEPS; LAP-3
UniProt Entry Name
AMPL_HUMAN
Uniprot Description
LAP3: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Belongs to the peptidase M17 family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: EC 3.4.11.5; Amino Acid Metabolism - arginine and proline; EC 3.4.11.1; Mitochondrial; Protease; Other Amino Acids Metabolism - glutathione
Chromosomal Location of Human Ortholog: 4p15.32
Cellular Component: nucleoplasm; focal adhesion; mitochondrion; cytoplasm; trans-Golgi network; nucleus
Molecular Function: metalloexopeptidase activity; manganese ion binding; aminopeptidase activity
Biological Process: proteolysis
Research Articles on LAP3
Similar Products
Product Notes
The LAP3 lap3 (Catalog #AAA9210457) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 89-119. The LAP3 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LAP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the LAP3 lap3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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