Western Blot (WB)
(Western blot analysis of extracts from K562 using LAMP1 antibody.)
Rabbit anti-Human LAMP1 Polyclonal Antibody | anti-LAMP1 antibody
LAMP1 Antibody
Gene Names
LAMP1; LAMPA; CD107a; LGP120
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
LAMP1; Polyclonal Antibody; LAMP1 Antibody; CD107 antigen like family member A; CD107 antigen-like family member A; CD107a; CD107a antigen; LAMP 1; LAMP-1; LAMP1_HUMAN; LAMPA; LGP120; lgpA; Lysosomal membrane glycoprotein 120KD; Lysosomal Associated Membrane Protein 1; Lysosome associated membrane glycoprotein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1; OTTHUMP00000040663; anti-LAMP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
LAMP1 antibody detects endogenous levels of total LAMP1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
417
Applicable Applications for anti-LAMP1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane. Endosome Membrane. Lysosome Membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Predicted Cross Reactivity
Rabbit, Dog
Similarity
Rabbit (82%), Dog (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from K562 using LAMP1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from K562 using LAMP1 antibody.)
Immunofluorescene (IF)
(MBS9606260 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9606260 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-LAMP1 antibody
Function: Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
Subunit Structure: (Microbial infection) Interacts with Lassa virus protein glycoprotein.
Post-translational Modifications: O-and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells.
Similarity: Belongs to the LAMP family.
Subunit Structure: (Microbial infection) Interacts with Lassa virus protein glycoprotein.
Post-translational Modifications: O-and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells.
Similarity: Belongs to the LAMP family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 125 kD
Predicted: 45 kDa
Predicted: 45 kDa
NCBI Official Full Name
lysosome-associated membrane glycoprotein 1
NCBI Official Synonym Full Names
lysosomal associated membrane protein 1
NCBI Official Symbol
LAMP1
NCBI Official Synonym Symbols
LAMPA; CD107a; LGP120
NCBI Protein Information
lysosome-associated membrane glycoprotein 1
UniProt Protein Name
Lysosome-associated membrane glycoprotein 1
Protein Family
UniProt Gene Name
LAMP1
UniProt Synonym Gene Names
LAMP-1; Lysosome-associated membrane protein 1
NCBI Description
The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq, Jul 2008]
Uniprot Description
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.Acts as a receptor for Lassa virus protein.
Research Articles on LAMP1
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Product Notes
The LAMP1 lamp1 (Catalog #AAA9606260) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LAMP1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LAMP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the LAMP1 lamp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAMP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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