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Western Blot (WB) (Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human LALBA Polyclonal Antibody | anti-LALBA antibody

LALBA Antibody (Center)

Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
LALBA; Polyclonal Antibody; LALBA Antibody (Center); Alpha-lactalbumin; Lactose synthase B protein; Lysozyme-like protein 7; LYZL7; anti-LALBA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This LALBA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the Central region of human LALBA.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
39-68
Sequence Length
142
Applicable Applications for anti-LALBA antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-LALBA Antibody (Center)at 1:2000 dilution + human breast lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(LALBA Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the LALBA antibody detected the LALBA protein (arrow).)

Western Blot (WB) (LALBA Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the LALBA antibody detected the LALBA protein (arrow).)

Immunohistochemistry (IHC)

(LALBA Antibody (Center) (MBS9202400)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LALBA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (LALBA Antibody (Center) (MBS9202400)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LALBA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-LALBA antibody
This gene encodes alpha-lactalbumin, a principal protein
of milk. Alpha-lactalbumin forms the regulatory subunit of the
lactose synthase (LS) heterodimer and beta
1,4-galactosyltransferase (beta4Gal-T1) forms the catalytic
component. Together, these proteins enable LS to produce lactose by
transfering galactose moieties to glucose. As a monomer,
alpha-lactalbumin strongly binds calcium and zinc ions and may
possess bactericidal or antitumor activity. A folding variant of
alpha-lactalbumin, called HAMLET, likely induces apoptosis in tumor
and immature cells.
Product Categories/Family for anti-LALBA antibody
References
Tolin, S., et al. FEBS J. 277(1):163-173(2010)
Rosner, H.I., et al. J. Mol. Biol. 394(2):351-362(2009)
Wang, J., et al. J. Dairy Sci. 91(12):4466-4476(2008)
Ramboarina, S., et al. J. Am. Chem. Soc. 130(46):15318-15326(2008)
Morozova, O.B., et al. J Phys Chem B 109(12):5912-5918(2005)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16225
NCBI Official Full Name
alpha-lactalbumin
NCBI Official Synonym Full Names
lactalbumin, alpha-
NCBI Official Symbol
LALBA
NCBI Protein Information
alpha-lactalbumin
UniProt Protein Name
Alpha-lactalbumin
Protein Family
UniProt Gene Name
LALBA
UniProt Synonym Gene Names
LYZL7
UniProt Entry Name
LALBA_HUMAN

NCBI Description

This gene encodes alpha-lactalbumin, a principal protein of milk. Alpha-lactalbumin forms the regulatory subunit of the lactose synthase (LS) heterodimer and beta 1,4-galactosyltransferase (beta4Gal-T1) forms the catalytic component. Together, these proteins enable LS to produce lactose by transfering galactose moieties to glucose. As a monomer, alpha-lactalbumin strongly binds calcium and zinc ions and may possess bactericidal or antitumor activity. A folding variant of alpha-lactalbumin, called HAMLET, likely induces apoptosis in tumor and immature cells. [provided by RefSeq, Jul 2008]

Uniprot Description

LALBA: Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins. Belongs to the glycosyl hydrolase 22 family.

Protein type: Apoptosis; Secreted; Transferase; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular space

Molecular Function: lactose synthase activity; calcium ion binding

Biological Process: cell-cell signaling; apoptosis; defense response to bacterium; signal transduction; lactose biosynthetic process

Research Articles on LALBA

Similar Products

Product Notes

The LALBA lalba (Catalog #AAA9202400) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 39-68. The LALBA Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LALBA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the LALBA lalba for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LALBA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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