Rabbit anti-Human KRT6B Polyclonal Antibody | anti-KRT6B antibody

KRT6B, ID (KRT6B, K6B, KRTL1, Keratin, type II cytoskeletal 6B, Cytokeratin-6B, Keratin-6B, Type-II keratin Kb10) (APC)

Cat. Num. AAA6314723

• Gene Names: KRT6B; K6B; PC2; PC4; CK6B; CK-6B; KRTL1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KRT6B; Polyclonal Antibody; ID (KRT6B; K6B; KRTL1; Keratin; type II cytoskeletal 6B; Cytokeratin-6B; Keratin-6B; Type-II keratin Kb10) (APC); Type-II keratin Kb10; anti-KRT6B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-KRT6B antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KRT6B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the Central region of human KRT6B.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KRT6B Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the KRT6B antibody detected the KRT6B protein (arrow).)

Immunohistochemistry (IHC)

(KRT6B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KRT6B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-KRT6B antibody

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq].

Product Categories/Family for anti-KRT6B antibody

Antibodies; Cytokeratin

References

Millar, E.K., et al. J. Clin. Oncol. 27(28):4701-4708(2009). Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006). Takahashi, K., et al. J. Biol. Chem. 270(31):18581-18592(1995) Rasmussen, H.H., et al. Electrophoresis 13(12):960-969(1992) Suo, Z., et al. Anticancer Res. 12 (6B), 2025-2031 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 119703753
• NCBI GeneID: 3854
• NCBI Accession #: NP_005546.2
• NCBI GenBank Nucleotide #: NM_005555.3
• UniProt Accession #: P04259; P48669
• Molecular Weight: 60,067 Da
• NCBI Official Full Name: keratin, type II cytoskeletal 6B
• NCBI Official Synonym Full Names: keratin 6B, type II
• NCBI Official Symbol: KRT6B
• NCBI Official Synonym Symbols: K6B; PC2; PC4; CK6B; CK-6B; KRTL1
• NCBI Protein Information: keratin, type II cytoskeletal 6B; cytokeratin 6B; cytokeratin-6B; keratin, epidermal, type II, K6B; keratin-like 1 (a type II keratin sequence); type-II keratin Kb10
• UniProt Protein Name: Keratin, type II cytoskeletal 6B
• Protein Family: Keratin
• UniProt Gene Name: KRT6B
• UniProt Synonym Gene Names: K6B; KRTL1; CK-6B; K6B
• UniProt Entry Name: K2C6B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Uniprot Description

KRT6B: Defects in KRT6B are a cause of pachyonychia congenita type 2 (PC2); also known as pachyonychia congenita Jackson-Lawler type. PC2 is an autosomal dominant ectodermal dysplasia characterized by hypertrophic nail dystrophy resulting in onchyogryposis (thickening and increase in curvature of the nail), palmoplantar keratoderma and hyperhidrosis, follicular hyperkeratosis, multiple epidermal cysts, absent/sparse eyebrow and body hair, and by the presence of natal teeth. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: keratin filament

Molecular Function: structural constituent of cytoskeleton

Biological Process: ectoderm development

Disease: Pachyonychia Congenita 4