Rabbit anti-Human, Mouse KRT4 Polyclonal Antibody | anti-KRT4 antibody

KRT4, CT (Keratin, Type II Cytoskeletal 4, Cytokeratin-4, CK-4, Keratin-4, K4, CYK4, Type-II Keratin Kb4)

Cat. Num. AAA625832

• Gene Names: KRT4; K4; CK4; CK-4; CYK4; FLJ31692
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: KRT4; Polyclonal Antibody; CT (Keratin; Type II Cytoskeletal 4; Cytokeratin-4; CK-4; Keratin-4; K4; CYK4; Type-II Keratin Kb4); Anti -KRT4; anti-KRT4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KRT4. Species Crossreactivity: mouse.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-KRT4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human KRT4 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KRT4 antibody (C-term) in mouse spleen tissue lysates (35ug/lane). KRT4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with KRT4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-231 cells using KRT4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KRT4 antibody

The protein is a member of the keratin family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13.

Product Categories/Family for anti-KRT4 antibody

Antibodies; Abs to Keratin; Cytokeratin

NCBI and Uniprot Product Information

• NCBI GI #: 331999954
• NCBI GeneID: 3851
• NCBI Accession #: NP_002263.3
• NCBI GenBank Nucleotide #: NM_002272.3
• UniProt Accession #: P19013; Q6GTR8; Q96LA7; Q9BTL1; Q6PIN2
• Molecular Weight: 57,285 Da
• NCBI Official Full Name: keratin, type II cytoskeletal 4
• NCBI Official Synonym Full Names: keratin 4
• NCBI Official Symbol: KRT4
• NCBI Official Synonym Symbols: K4; CK4; CK-4; CYK4; FLJ31692
• NCBI Protein Information: keratin, type II cytoskeletal 4; cytokeratin 4; type-II keratin Kb4
• UniProt Protein Name: Keratin, type II cytoskeletal 4
• Protein Family: Keratin
• UniProt Gene Name: KRT4
• UniProt Synonym Gene Names: CYK4
• UniProt Entry Name: K2C4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]

Uniprot Description

K4: Heterotetramer of two type I and two type II keratins. Keratin-4 is generally associated with keratin-13. Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa. Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: intermediate filament cytoskeleton; cell surface; keratin filament; intermediate filament; nucleus

Molecular Function: protein binding; structural molecule activity

Biological Process: epithelial cell differentiation; cytoskeleton organization and biogenesis; negative regulation of epithelial cell proliferation

Disease: White Sponge Nevus 1